Filters
Sensor Target Type Specificity Sensor Name Sensor Type Analysis Technique IV Propertiessort descending Organism Binding Protein Fluorophore PIs Sequence Addgene Link
Metabolites FRET-Based sensor confocal microscopy Kd: 107 uM HeLa cells, Astrocytes, HEK293 cells, hippocampal neurons PdhR mTFP, Venus Felipe Barros Available
Metabolites

diacylglycerol

Daglas

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase Specific MDCK cells, NIH 3T3 C1 domain of PKCßII as DAG binding domain CFP, YFP Matsuda M
Kinase Activity

CyclinB-Cdk1

CyclinB-Cdk1 sensor

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase Specific HeLa cells, Xenopus, MEF, hTert-RPE1 cells Polo box domain (PBD) of PIk1 CFP(mCerulean) , YFP(YPet) Pines J
Kinase Activity

epidermal growth factor receptor (EGFR)

ECaus

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase Specific CHO-EGFR cells Substrate, SH2 domain CFP, YFP Umezawa Y
Kinase Activity

Abl

Crk-based reporter

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific (tyrosine) NIH 3T3, MEF cells complete chicken c-CrkII gene eCFP, eYFP (citrine) Tsien RY
Kinase Activity

Abl

Src indicator

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific (tyrosine) NIH 3T3, HeLa Cells Rous sarcoma virus Src gene (residues148–245) eCFP, eYFP (citrine) Tsien RY
Kinase Activity

Abl

Picchu

FRET-based biosensors fluorescence microscopy Kinase Specific (tyrosine) HeLa cells, Cos7/E3 Src-homology (SH) 2 of Crk II or phosphotyrosine-binding (PTB) eYFP, CFP Matsuda M
Kinase Activity

Abl

Picchu-Z/EGFR-Z

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific (tyrosine) Cos1 and HT1080 and NIH3T3 and A431 Src-homology (SH) 2 and SH3 from Crk II YFP, CFP Matsuda M
Kinase Activity

epidermal growth factor receptor (EGFR)

Bescell/ BBD-Ecat

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase specific, selective to BDNF MCF-7 cells BDNF binding to BBD-Ecat domain CFP, YFP
Kinase Activity

Abl

EGFR indicator

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific, sensitive to concentration of EGF B82, NIH 3T3, HeLa cells SH2 domain (residues 374–465) from mouse p52 Shc eCFP, eYFP (citrine) Tsien RY
Metabolites

cGMP

Cygnus

FRET-based biosensors FLIM, confocal fluorescence microscopy, spectroscopy pH-sensitive PC12 cells, HEK 293T cells, HeLa cells cGMP binding from PDE5 mTagBFP, sREACh(dark) Oka K
Metabolites

arginine

FRET arginine reporter

FRET-based biosensors confocal flourescence microscopy, spectroscopy pH-sensitive, ornithine Kd-9.2 mM, Arg Kd-3.2 mM E. coli BL21(DE3)Gold, Arabidopsis thalina Protoplasts and roots bacterial glutamine binding protein (QBP) from E. coli with T70A and D157N eCFP, Citrine (shortened) Ludewig U
Ions

Cl-

Cl-sensor

FRET-based biosensors fluorescence microscopy, spectroscopy pH-specific/sensitive CHO-K1 cells, HEK cells, hippocampal neurons, photoreceptors from rat retina peptide linker mutated eYFP, CFP Bregestovski P Plain text icon test_sequence_ bregestovski.txt
Ions

Cl-

Clomeleon

FRET-based biosensors confocal flourescence microscopy PKD1 (K467A) Kd: .196 µM, WT PKD1 - .012, µM, PKD1 (K467R) - .009 µM E.coli, HEK-293 cells PDK1 PH domain green fluorescent protein (GFP-) tagged versions of wild-type PDK1 van Aalten DM
Kinase Activity

epidermal growth factor receptor (EGFR)

EGFR-ECFP/PTV-EYFP

FRET-based biosensors FLIM, fluorescence microscopy PTB domain (KD, 140 nM to 5.3 µM) MCF-7 cells Phosphotyrosine (PTB) domain of human Shc eCFP, eYFP (citrine) Bastiaens PI
Kinase Activity

epidermal growth factor receptor (EGFR)

EGFR-ECFP/PTV-EYFP

FRET-based biosensors FLIM, fluorescence microscopy PTB domain (KD, 140 nM to 5.3 µM) COS-7 cells PTB domain eCFP, eYFP Bastiaens PI
Metabolites

cAMP

Epac-camps

FRET-based biosensors spectroscopy, fluorescence microscopy sensitive cAMP indicators Sf9 cells, CHO-A2B, TsA201, HEK293, COS-7 cells cAMP binding eCFP, eYFP Lohse MJ
Metabolites

cAMP

PKA-camps

FRET-based biosensors spectroscopy, fluorescence microscopy sensitive cAMP indicators Sf9 cells, CHO-A2B, TsA201, HEK293, COS-7 cells cAMP binding eCFP, eYFP Lohse MJ
Metabolites

diacylglycerol

CKAR

FRET-based biosensors fluorescence microscopy, spectroscopy Sensitive to calyculcin A and PKC HeLa cells, MDCK cells FHA2 domain of Rad53p mCFP, mYFP Newton AC
Kinase Activity

Extracellular signal-regulated kinase (ERK)

Erkus

FRET-based biosensors confocal flourescence microscopy Sensitive to ERK activity MCF-7 cells FHA2 domain and ERK docking domain eCFP, eYFP Umezawa Y
Kinase Activity

Extracellular signal-regulated kinase (ERK)

EAS

FRET-based biosensors spectroscopy Sensitive to ERK activity BL21(DE3) cells, NIH 3T3 Serine-Proline (SP) binding, either D-domain or DEF domain eCFP, eYFP Alberola-Ila J
Kinase Activity

Extracellular signal-regulated kinase (ERK)

EKAR

FRET-based biosensors FLIM, fluorescence microscopy Sensitive to p38 and JNK and ERK activities HEK293 cells, pyramidal neurons, COS-7 cells Phospho-binding domain and ERK docking domain eGFP , mRFP1 or CFP , YFP Svoboda K

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