Filters
Sensor Target Type Specificity Sensor Name Sensor Typesort ascending Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs Sequence Addgene Link
Ions

Ca2+

R-GECO

Single FP-based extrinsic biosensors Tsien RY, Campbell RE
Ions

Ca2+

jRGECO1a

Single FP-based extrinsic biosensors Cultured neurons mApple Kim DS
Metabolites FRET-Based sensor confocal microscopy Kd 1: 8 uM/ Kd 2: 800 uM HeLa cells, Astrocytes, HEK293 cells, hippocampal neurons LldR mTFP, Venus Felipe Barros Available
Metabolites FRET-Based sensor confocal microscopy Kd: 107 uM HeLa cells, Astrocytes, HEK293 cells, hippocampal neurons PdhR mTFP, Venus Felipe Barros Available
Ions

Ca2+

FIP-CBSMTN-XL

FRET-based biosensors Miyawaki A, Persechini A,, Ikura M
Metabolites

diacylglycerol

CKAR

FRET-based biosensors fluorescence microscopy, spectroscopy Sensitive to calyculcin A and PKC HeLa cells, MDCK cells FHA2 domain of Rad53p mCFP, mYFP Newton AC
Redox and Voltage

redox

HSP-FRET

FRET-based biosensors CFP, YFP Schumacker PT
Kinase Activity

Abl

Crk-based reporter

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific (tyrosine) NIH 3T3, MEF cells complete chicken c-CrkII gene eCFP, eYFP (citrine) Tsien RY
Kinase Activity

Protein kinase D

DKAR

FRET-based biosensors COS-7 cells, HeLa Cells mCFP, mYFP Newton AC
Metabolites

tryptophan

FLIPW sensors

FRET-based biosensors Frommer WB
Ions

Ca2+

TN-XXL

FRET-based biosensors Griesbeck O, Miyawaki A, Persechini A, Ikura M
Metabolites

glucose

FLIPglu sensors

FRET-based biosensors confocal flourescence microscopy, spectroscopy KD 600µM Saccharomyces, arabidopsis, HEK293T, COS-7 cells mature glucose/galactose-binding protein MglB eCFP, eYFP Frommer WB, Frommer WB, Frommer WB
Redox and Voltage

redox

Redoxfluor

FRET-based biosensors CHO cells Cerulean, Citrine Sakai Y
Kinase Activity

Abl

EGFR indicator

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific, sensitive to concentration of EGF B82, NIH 3T3, HeLa cells SH2 domain (residues 374–465) from mouse p52 Shc eCFP, eYFP (citrine) Tsien RY
Kinase Activity

Src

Src reporter

FRET-based biosensors MCF-7 cells CFP, YFP Umezawa Y
Ions

Cl-

Clomeleon

FRET-based biosensors confocal flourescence microscopy PKD1 (K467A) Kd: .196 µM, WT PKD1 - .012, µM, PKD1 (K467R) - .009 µM E.coli, HEK-293 cells PDK1 PH domain green fluorescent protein (GFP-) tagged versions of wild-type PDK1 van Aalten DM
Metabolites

glucose

AcGFP1-GBPcys-mCherry

FRET-based biosensors Ye K
Kinase Activity

Abl

Src indicator

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific (tyrosine) NIH 3T3, HeLa Cells Rous sarcoma virus Src gene (residues148–245) eCFP, eYFP (citrine) Tsien RY
Kinase Activity

Src

Srcus

FRET-based biosensors HeLa cells, MEF cells CFP, YFP Chien S
Ions

Cl-

Cl-sensor

FRET-based biosensors fluorescence microscopy, spectroscopy pH-specific/sensitive CHO-K1 cells, HEK cells, hippocampal neurons, photoreceptors from rat retina peptide linker mutated eYFP, CFP Bregestovski P Plain text icon test_sequence_ bregestovski.txt
Metabolites

glutamate

FLIPE sensors

FRET-based biosensors confocal flourescence microscopy, spectroscopy KD 630 nM Rat hippocampal cells, PC12 cells glutamate/aspartate binding protein ybeJ from Escherichia coli eCFP, Venus Frommer WB, Frommer WB
Kinase Activity

Abl

Picchu

FRET-based biosensors fluorescence microscopy Kinase Specific (tyrosine) HeLa cells, Cos7/E3 Src-homology (SH) 2 of Crk II or phosphotyrosine-binding (PTB) eYFP, CFP Matsuda M
Mechanical Force

Mechanical strain

VinTS

FRET-based biosensors confocal microscopy BAECs, MEF cells, HEK293 cells mTFP1, venus(A206K) Schwartz MA
Ions

Cu+

Ace1-FRET

FRET-based biosensors fluorescence microscopy, spectroscopy Cu+ sensitive Yeast cells, E.coli Copper (I) binding domain CFP, YFP He C
Metabolites

glutamate

GluSnFR

FRET-based biosensors Tsien RY

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