Filters
Sensor Target Type Specificity Sensor Name Sensor Typesort descending Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs
Metabolites

cGMP

CGYs

FRET-based biosensors confocal flourescence microscopy CHO-K1 cells, HEK-293 cells PKG-Ia binding eCFP, eYFP Umezawa Y
Protein Activation and Conformation

Rho family GTPase activation

Raichu-RBD

FRET-based biosensors Matsuda M
Ions

Zn2+

Cys2His2

FRET-based biosensors Neurons Palmer AE
Kinase Activity

Protein kinase B/Akt

BKAR

FRET-based biosensors NIH 3T3 mCFP, mYFP Newton AC
Metabolites

cGMP

cGES sensors

FRET-based biosensors confocal flourescence microscopy HEK-293 cells GAF-B domain of PDE2A and GAF-A domain of PDE5A eCFP, eYFP Lohse MJ
Protein Activation and Conformation

Rho family GTPase activation

Raichu-RalA

FRET-based biosensors COS-7 cells CFP, YFP Matsuda M
Ions

Zn2+

ZapCY

FRET-based biosensors HELA cells (Cytosol, Golgi, ER) Palmer AE
Metabolites

PtdIns(3,4,5)P3

CAY

FRET-based biosensors Swiss 3T3 fibroblasts Allen PG
Kinase Activity

Protein kinase B/Akt

Aktus

FRET-based biosensors CHO cells CFP, YFP Umezawa Y
Metabolites

cGMP

cGi sensors

FRET-based biosensors spectroscopy, fluorescence microscopy cGMP concentration 1-2 µM HEK293 cells cGMP-binding sites cNMP-BD (cGKI) or GAF (PDE5) eCFP, eYFP Koesling D
Protein Activation and Conformation

Rho family GTPase activation

RhoA biosensor commentary

FRET-based biosensors Hahn KM, Hahn KM
Ions

Cd2+

Cd-FRET-2

FRET-based biosensors Merkx M
Metabolites

PtdIns(3,4,5)P3

Pippi-PI(3,4,5)P3

FRET-based biosensors MDCK cells Matsuda M
Kinase Activity

Protein kinase B/Akt

Akind

FRET-based biosensors COS-7 cells CFP, YFP Matsuda M
Metabolites

cGMP

Cygnus

FRET-based biosensors FLIM, confocal fluorescence microscopy, spectroscopy pH-sensitive PC12 cells, HEK 293T cells, HeLa cells cGMP binding from PDE5 mTagBFP, sREACh(dark) Oka K
Protein Activation and Conformation

Wiskott -Aldrich syndrome protein (WASP) conformation

Cdc42-GEF sensors

FRET-based biosensors NIH 3T3 cells eCFP, eYFP Rosen MK
Ions

Zn2+

ZinCh

FRET-based biosensors Merkx M
Metabolites

PtdIns(3,4,5)P3

Fllip

FRET-based biosensors CHO cells Umezawa Y
Kinase Activity

Protein kinase B/Akt

GFP-PKB-RFP

FRET-based biosensors NIH 3T3 GFP, RFP Larijani B
Metabolites

citrate

CIT sensors

FRET-based biosensors fluorescence microscopy Kd range - 8 µM - 1765 µM Kd: CFP-CitA6-130-Venus - 8 µM COS cells, arabidopsis, yeast, e.coli citrate binding of the Klebsiella pneumoniae histidine sensor kinase CitA venus, CFP Zamboni N
Metabolites

PtdIns(3,4,5)P3

Pippi-PI(3,4,5)P3

FRET-based biosensors COS-7 cells Matsuda M
Kinase Activity

Protein kinase B/Akt

ReAktion

FRET-based biosensors NIH 3T3 CFP, YFP Zhang J
Metabolites

diacylglycerol

Daglas

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase Specific MDCK cells, NIH 3T3 C1 domain of PKCßII as DAG binding domain CFP, YFP Matsuda M
Kinase Activity

Protein kinase B/Akt

AktAR

FRET-based biosensors NIH 3T3 Cerulean, cpV E172 Zhang J
Metabolites

ribose

FLIPrib sensors

FRET-based biosensors Lalonde S

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