Filters
Sensor Target Typesort descending Specificity Sensor Name Sensor Type Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs Addgene Link
Kinase Activity

Src

Src reporter

FRET-based biosensors MCF-7 cells CFP, YFP Umezawa Y
Kinase Activity

Abl

Src indicator

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific (tyrosine) NIH 3T3, HeLa Cells Rous sarcoma virus Src gene (residues148–245) eCFP, eYFP (citrine) Tsien RY
Kinase Activity

Src

Srcus

FRET-based biosensors HeLa cells, MEF cells CFP, YFP Chien S
Kinase Activity

Abl

Picchu

FRET-based biosensors fluorescence microscopy Kinase Specific (tyrosine) HeLa cells, Cos7/E3 Src-homology (SH) 2 of Crk II or phosphotyrosine-binding (PTB) eYFP, CFP Matsuda M
Kinase Activity

Abl

Picchu-Z/EGFR-Z

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific (tyrosine) Cos1 and HT1080 and NIH3T3 and A431 Src-homology (SH) 2 and SH3 from Crk II YFP, CFP Matsuda M
Kinase Activity

ATM-Protein Kinase

ATOMIC

FRET-based biosensors confocal flourescence microscopy irreversible HeLa cells, NIH 3T3, AT4Bi Rad53 FHA2 (Chk2) and peptide with T68 phosphorylation site CFP, YFP Hunter T
Kinase Activity

Aurora B kinase

aurora B sensor

FRET-based biosensors confocal flourescence microscopy Xenopus S3, HeLa Cells, DU145, DLD21 reversible binding to an FHA2 phospho-threonine binding domain CFP, YFP Kapoor TM
Kinase Activity

CyclinB-Cdk1

CyclinB-Cdk1 sensor

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase Specific HeLa cells, Xenopus, MEF, hTert-RPE1 cells Polo box domain (PBD) of PIk1 CFP(mCerulean) , YFP(YPet) Pines J
Mechanical Force

Mechanical strain

VinTS

FRET-based biosensors confocal microscopy BAECs, MEF cells, HEK293 cells mTFP1, venus(A206K) Schwartz MA
Mechanical Force

Mechanical strain

stFRET

FRET-based biosensors Dictyostelium , HS1, AX2 GFP, eGFP Uyeda TQ
Mechanical Force

Mechanical strain

PriSSM (PRIM-based)

FRET-based biosensors BAECs, HEK cells Cerulean, Venus Sachs F
Mechanical Force

Mechanical strain

sstFRET

FRET-based biosensors confocal microscopy HEK-293, 3T3, C.Elegans Cerulean, Venus Sachs F
Metabolites

histidine

FLIP-HisJ/cpHisJ194

FRET-based biosensors Ito T
Metabolites

PtdIns(3,5)P2

GFP-PHPDK1

Translocation-based biosensors Alessi DR
Metabolites

1,2-diacylglycerol

DAGR (FRET)

Translocation-based biosensors Newton AC
Metabolites

inositol 1,4,5-trisphosphate

LIBRA

FRET-based biosensors Mikoshiba K
Metabolites

PtdIns(3,5)P2

YFP-PHTAPP1

Translocation-based biosensors Alessi DR
Metabolites

arabinose

FLIParaF.Ec-250n

FRET-based biosensors spectroscopy Kd-230nM ± 18 nM E. coli BL21-Gold(DE3) E.coli K12 araF eCFP, Venus Frommer WB Available
Metabolites

inositol 1,4,5-trisphosphate

FIRE1-3

FRET-based biosensors Mignery GA
Metabolites

PtdIns(3,5)P2

GFP-Svp1p

Translocation-based biosensors Lemmon MA
Metabolites

arginine

FRET arginine reporter

FRET-based biosensors confocal flourescence microscopy, spectroscopy pH-sensitive, ornithine Kd-9.2 mM, Arg Kd-3.2 mM E. coli BL21(DE3)Gold, Arabidopsis thalina Protoplasts and roots bacterial glutamine binding protein (QBP) from E. coli with T70A and D157N eCFP, Citrine (shortened) Ludewig U
Metabolites

inositol 1,4,5-trisphosphate

IRIS-1

FRET-based biosensors Tojyo Y
Metabolites

PtdIns(3,5)P2

GFP-PHBtk

Translocation-based biosensors Balla T
Metabolites

amino acids

FLIPs for amino acids

FRET-based biosensors fluorescence microscopy, spectroscopy cross-binding of other aa Yeast cells circularly permutated PBPs eCFP, Venus Ito T
Metabolites

leucine

FLIP-LivJ/cpLivJ261

FRET-based biosensors Ito T

Pages