Filters
Sensor Target Type Specificity Sensor Name Sensor Typesort descending Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs
Metabolites

inositol 1,4,5-trisphosphate

FIRE1-3

FRET-based biosensors Mignery GA
Redox and Voltage

voltage

VSFPs

FRET-based biosensors Knöpfel T, Knöpfel T, Knöpfel T, Knöpfel T
Kinase Activity

epidermal growth factor receptor (EGFR)

Bescell/ BBD-Ecat

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase specific, selective to BDNF MCF-7 cells BDNF binding to BBD-Ecat domain CFP, YFP
Metabolites

amino acids

FLIPs for amino acids

FRET-based biosensors fluorescence microscopy, spectroscopy cross-binding of other aa Yeast cells circularly permutated PBPs eCFP, Venus Ito T
Protein Activation and Conformation

CaMKII conformation

CamuiαamCYCaMIIα

FRET-based biosensors two-photon fluorescence microscopy HeLa cells YFP(Venus) , CFP (S175G) Schultz C, Hayashi Y
Metabolites

inositol 1,4,5-trisphosphate

IRIS-1

FRET-based biosensors Tojyo Y
Redox and Voltage

voltage

Mermaid

FRET-based biosensors Neuro2A cells CFP, YFP Miyawaki A
Kinase Activity

epidermal growth factor receptor (EGFR)

EGFR-ECFP/PTV-EYFP

FRET-based biosensors FLIM, fluorescence microscopy PTB domain (KD, 140 nM to 5.3 µM) MCF-7 cells Phosphotyrosine (PTB) domain of human Shc eCFP, eYFP (citrine) Bastiaens PI
Metabolites

ATP

ATeams

FRET-based biosensors spectroscopy, fluorescence microscopy ATeams Kd - 7.4 µM and 1.7 (AT3.10), 14 µM and 2.0 (AT3.10MGK), 1.2 mM and 2.1 (AT1.03YEMK), and 3.3 mM and 2.1 (AT1.03), respectively. HeLa cells, NIH 3T3 ? subunit from Bacillus subtilis FoF1-ATP synthase CFP (mseCFP), YFP (cp173-mVenus) Noji H
Protein Activation and Conformation

ERK conformation

Miu2

FRET-based biosensors HeLa cells, COS-7 cells, 293F CFP, YFP, RFP(Dropna) Matsuda M
Metabolites

leucine

FLIP-LivJ/cpLivJ261

FRET-based biosensors Ito T
Redox and Voltage

voltage

FlaSh-CFP/YFP

FRET-based biosensors salamander retinal cones, on-bipolar cells, horizontal cells, wide-field amacrine cells eGFP, GFPuv, and wtGFP, eCFP and eYFP Isacoff EY
Kinase Activity

epidermal growth factor receptor (EGFR)

EGFR-ECFP/PTV-EYFP

FRET-based biosensors FLIM, fluorescence microscopy PTB domain (KD, 140 nM to 5.3 µM) COS-7 cells PTB domain eCFP, eYFP Bastiaens PI
Protein Activation and Conformation

Estrogen receptor ligand binding domain conformation

CEY

FRET-based biosensors COS-7 cells, Sf9 cells, Hf7c cells ER-Ligand binding domain seCFP, eYFP Lannigan DA
Metabolites

maltose

FLIPmal sensors

FRET-based biosensors confocal flourescence microscopy arabinose FLIP Kd-200 nM,maltose FLIP Kd-40 µM Escherichia coli BL21-Gold(DE3) cells periplasmic binding protein for arabinose or maltose, E. coli high-affinity L-arabinose binding protein AraF eCFP, eYFP (venus) Frommer WB
Kinase Activity

Extracellular signal-regulated kinase (ERK)

Erkus

FRET-based biosensors confocal flourescence microscopy Sensitive to ERK activity MCF-7 cells FHA2 domain and ERK docking domain eCFP, eYFP Umezawa Y
Kinase Activity

Extracellular signal-regulated kinase (ERK)

EAS

FRET-based biosensors spectroscopy Sensitive to ERK activity BL21(DE3) cells, NIH 3T3 Serine-Proline (SP) binding, either D-domain or DEF domain eCFP, eYFP Alberola-Ila J
Metabolites

BAI2 (a furanosyl borate diester)

CLPY

FRET-based biosensors fluorescence microscopy 98 kDa (CLPY), BAI2 Kd 270 nM BL21 (luxS-) cells transformed with pQE30-CLPY or LuxP-mutant constructs LuxP-BAI2 binding CFP, YFP Sayre R
Protein Activation and Conformation

G protein activation

FP-Gα/FP-Gβ(γ)

FRET-based biosensors HeLa cells, HEK cells, HEK293T, Dictyostelium discoideum cells, FRT cells, haploid yeast cells YFP(Gaq-mYFP) and CFP(mTq), YFP and CFP, eYFP Gadella TW Jr, Lohse MJ, Bünemann M, Gilman AG, Bünemann M, Devreotes P, Scarlata S, Simon MI
Metabolites

NO

FRET-MT

FRET-based biosensors Levitan ES
Kinase Activity

Extracellular signal-regulated kinase (ERK)

EKAR

FRET-based biosensors FLIM, fluorescence microscopy Sensitive to p38 and JNK and ERK activities HEK293 cells, pyramidal neurons, COS-7 cells Phospho-binding domain and ERK docking domain eGFP , mRFP1 or CFP , YFP Svoboda K
Metabolites

BAI2 (a furanosyl borate diester)

LuxP-FRET

FRET-based biosensors spectroscopy, fluorescence microscopy BAI2 Kd 270 nM Escherichia coli BL21 (luxS-), V. harVeyi strains BB120 (wild-type), MM30 (luxS-), MM32 (luxS-, luxN-) LuxP-BAI2 binding CFP, YFP Sayre R
Metabolites

Phosphatidic acid

Pii

FRET-based biosensors Kiyokawa E
Kinase Activity

Extracellular signal-regulated kinase (ERK)

Miu2

FRET-based biosensors HeLa cells CFP, YFP Matsuda M
Metabolites

cAMP

FP-PKARII/FP-PKAcat

FRET-based biosensors eBFP and GFP or CFP and YFP Pozzan T, Pozzan T

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