Filters
Sensor Target Typesort ascending Specificity Sensor Name Sensor Type Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs
Metabolites

amino acids

FLIPs for amino acids

FRET-based biosensors fluorescence microscopy, spectroscopy cross-binding of other aa Yeast cells circularly permutated PBPs eCFP, Venus Ito T
Metabolites

inositol 1,4,5-trisphosphate

IRIS-1

FRET-based biosensors Tojyo Y
Metabolites

PtdIns(3,5)P2

GFP-PHBtk

Translocation-based biosensors Balla T
Metabolites

ATP

ATeams

FRET-based biosensors spectroscopy, fluorescence microscopy ATeams Kd - 7.4 µM and 1.7 (AT3.10), 14 µM and 2.0 (AT3.10MGK), 1.2 mM and 2.1 (AT1.03YEMK), and 3.3 mM and 2.1 (AT1.03), respectively. HeLa cells, NIH 3T3 ? subunit from Bacillus subtilis FoF1-ATP synthase CFP (mseCFP), YFP (cp173-mVenus) Noji H
Metabolites

leucine

FLIP-LivJ/cpLivJ261

FRET-based biosensors Ito T
Metabolites

PtdIns(3,5)P2

GFP-Ent3p

Translocation-based biosensors Letourneur F
Metabolites

ATP:ADP ratio

Perceval

Single FP-based extrinsic biosensors Yellen G
Metabolites

maltose

FLIPmal sensors

FRET-based biosensors confocal flourescence microscopy arabinose FLIP Kd-200 nM,maltose FLIP Kd-40 µM Escherichia coli BL21-Gold(DE3) cells periplasmic binding protein for arabinose or maltose, E. coli high-affinity L-arabinose binding protein AraF eCFP, eYFP (venus) Frommer WB
Metabolites

PtdIns(4,5)P2

GFP-ARNO

Translocation-based biosensors Czech MP
Metabolites

BAI2 (a furanosyl borate diester)

CLPY

FRET-based biosensors fluorescence microscopy 98 kDa (CLPY), BAI2 Kd 270 nM BL21 (luxS-) cells transformed with pQE30-CLPY or LuxP-mutant constructs LuxP-BAI2 binding CFP, YFP Sayre R
Metabolites

NO

FRET-MT

FRET-based biosensors Levitan ES
Metabolites

PtdIns(4,5)P2

GFP-Cytohesin-1

Translocation-based biosensors Czech MP
Metabolites

BAI2 (a furanosyl borate diester)

LuxP-FRET

FRET-based biosensors spectroscopy, fluorescence microscopy BAI2 Kd 270 nM Escherichia coli BL21 (luxS-), V. harVeyi strains BB120 (wild-type), MM30 (luxS-), MM32 (luxS-, luxN-) LuxP-BAI2 binding CFP, YFP Sayre R
Metabolites

Phosphatidic acid

Pii

FRET-based biosensors Kiyokawa E
Metabolites

PtdIns(4,5)P2

GFP-PHOSH2

Translocation-based biosensors confocal microscopy HeLa cells, yeast cells eGFP Lemmon MA
Metabolites

cAMP

FP-PKARII/FP-PKAcat

FRET-based biosensors eBFP and GFP or CFP and YFP Pozzan T, Pozzan T
Metabolites

Phosphatidylserine

GFP-Lact-C2

Translocation-based biosensors Grinstein S
Metabolites

PtdIns(4,5)P2

PHOSBP

Translocation-based biosensors confocal microscopy COS-7 cells GFP, mRFP Balla T, Munro S
Metabolites

cAMP

CFP–Epac–YFP

FRET-based biosensors fluorescence microscopy highly sensitive cAMP indicator, Kd of 2.3 µM human A431 cells, Rat-1 and NIH3T3 fibroblasts, mouse GE11 epithelial cells, mouse N1E-115 neuroblastoma, human MCF7 breast carcinoma cells cAMP binding CFP, YFP Jalink K
Metabolites

PtdIns(3,4,5)P3

GFP-ARNO

Translocation-based biosensors Czech MP
Metabolites

PtdIns(4,5)P2

GFP-PHFAPP

Translocation-based biosensors confocal microscopy HeLa cells, yeast cells eGFP Lemmon MA
Metabolites

cAMP

ICUEs

FRET-based biosensors eCFP, Citrine Zhang J, Zhang J, Lefkowitz RJ
Metabolites

PtdIns(3,4,5)P3

GFP-PHGRP1

Translocation-based biosensors confocal microscopy PC12 cells GFP Cullen PJ, Czech MP
Metabolites

PtdIns(4,5)P2

CAY

FRET-based biosensors Swiss 3T3 fibroblasts Allen PG
Metabolites

cAMP

Epac-camps

FRET-based biosensors spectroscopy, fluorescence microscopy sensitive cAMP indicators Sf9 cells, CHO-A2B, TsA201, HEK293, COS-7 cells cAMP binding eCFP, eYFP Lohse MJ

Pages