Filters
Sensor Target Type Specificity Sensor Namesort descending Sensor Type Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs
Metabolites

cGMP

Cygnus

FRET-based biosensors FLIM, confocal fluorescence microscopy, spectroscopy pH-sensitive PC12 cells, HEK 293T cells, HeLa cells cGMP binding from PDE5 mTagBFP, sREACh(dark) Oka K
Protein Activation and Conformation

Annexin A4 self-association

CYNEX4

FRET-based biosensors confocal flourescence microscopy, FRAP HeLa cells, N1E-115, MDCK2 neuroblastoma cells Annexin A4 (Ca2+ regulated phospholated) eCFP, eYFP Schultz C
Metabolites

PtdIns(4,5)P2:Ins(1,4,5) P3 dynamics

CYPHR (FRET: PLC activity)

Translocation-based biosensors Shapiro L
Metabolites

1,2-diacylglycerol

Cys1-GFP

Translocation-based biosensors Meyer T
Ions

Zn2+

Cys2His2

FRET-based biosensors Neurons Palmer AE
Metabolites

diacylglycerol

Daglas

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase Specific MDCK cells, NIH 3T3 C1 domain of PKCßII as DAG binding domain CFP, YFP Matsuda M
Metabolites

diacylglycerol

DAGR

FRET-based biosensors confocal flourescence microscopy MDCK cells Cysteine-rich domain (CRD) CFP, YFP Umezawa Y
Metabolites

1,2-diacylglycerol

DAGR (FRET)

Translocation-based biosensors Newton AC
Ions

pH

deGFP1-4

Single FP-based intrinsic biosensors Remington SJ
Kinase Activity

Protein kinase D

DKAR

FRET-based biosensors COS-7 cells, HeLa Cells mCFP, mYFP Newton AC
Enzyme Activity (Non-Kinase)

various proteases

donor FP-substrate-acceptor FP

FRET-based biosensors HeLa cells eCFP, eBFP Luo Y
Ions

pH

E2GFP

Single FP-based intrinsic biosensors Beltram F
Kinase Activity

Extracellular signal-regulated kinase (ERK)

EAS

FRET-based biosensors spectroscopy Sensitive to ERK activity BL21(DE3) cells, NIH 3T3 Serine-Proline (SP) binding, either D-domain or DEF domain eCFP, eYFP Alberola-Ila J
Ions

Zn2+

eCALWYs

FRET-based biosensors Pancreatic beta-cells Rutter GA, Merkx M
Kinase Activity

epidermal growth factor receptor (EGFR)

ECaus

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase Specific CHO-EGFR cells Substrate, SH2 domain CFP, YFP Umezawa Y
Ions

pH

ECFP

Single FP-based intrinsic biosensors Tsien RY, Verkman AS
Ions

Hg2+

eGFP205C

Single FP-based intrinsic biosensors Sagermann M
Kinase Activity

Abl

EGFR indicator

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific, sensitive to concentration of EGF B82, NIH 3T3, HeLa cells SH2 domain (residues 374–465) from mouse p52 Shc eCFP, eYFP (citrine) Tsien RY
Kinase Activity

epidermal growth factor receptor (EGFR)

EGFR reporter

FRET-based biosensors HeLa cells, NIH 3T3 CFP, YFP Tsien RY
Kinase Activity

epidermal growth factor receptor (EGFR)

EGFR-ECFP/PTV-EYFP

FRET-based biosensors FLIM, fluorescence microscopy PTB domain (KD, 140 nM to 5.3 µM) MCF-7 cells Phosphotyrosine (PTB) domain of human Shc eCFP, eYFP (citrine) Bastiaens PI
Kinase Activity

epidermal growth factor receptor (EGFR)

EGFR-ECFP/PTV-EYFP

FRET-based biosensors FLIM, fluorescence microscopy PTB domain (KD, 140 nM to 5.3 µM) COS-7 cells PTB domain eCFP, eYFP Bastiaens PI
Kinase Activity

Extracellular signal-regulated kinase (ERK)

EKAR

FRET-based biosensors FLIM, fluorescence microscopy Sensitive to p38 and JNK and ERK activities HEK293 cells, pyramidal neurons, COS-7 cells Phospho-binding domain and ERK docking domain eGFP , mRFP1 or CFP , YFP Svoboda K
Metabolites

cAMP

Epac-camps

FRET-based biosensors spectroscopy, fluorescence microscopy sensitive cAMP indicators Sf9 cells, CHO-A2B, TsA201, HEK293, COS-7 cells cAMP binding eCFP, eYFP Lohse MJ
Kinase Activity

Extracellular signal-regulated kinase (ERK)

Erkus

FRET-based biosensors confocal flourescence microscopy Sensitive to ERK activity MCF-7 cells FHA2 domain and ERK docking domain eCFP, eYFP Umezawa Y
Ions

pH

EYFP

Single FP-based intrinsic biosensors Tsien RY, Verkman AS

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