Filters
Sensor Target Type Specificity Sensor Name Sensor Typesort descending Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs Addgene Link
Mechanical Force

Mechanical strain

stFRET

FRET-based biosensors Dictyostelium , HS1, AX2 GFP, eGFP Uyeda TQ
Ions

Cu+

Mac1-FRET

FRET-based biosensors fluorescence microscopy, spectroscopy Cu+ sensitive Yeast cells, E.coli Copper (I) binding domain CFP, YFP He C
Metabolites

glutamate

FLIP-cpGltI210

FRET-based biosensors Ito T
Kinase Activity

ATM-Protein Kinase

ATOMIC

FRET-based biosensors confocal flourescence microscopy irreversible HeLa cells, NIH 3T3, AT4Bi Rad53 FHA2 (Chk2) and peptide with T68 phosphorylation site CFP, YFP Hunter T
Metabolites

PtdIns(3,4)P2

Pippi-PI(3,4)P2

FRET-based biosensors MDCK cells Matsuda M
Mechanical Force

Mechanical strain

PriSSM (PRIM-based)

FRET-based biosensors BAECs, HEK cells Cerulean, Venus Sachs F
Ions

Cu+

Amt1-FRET

FRET-based biosensors fluorescence microscopy, spectroscopy Cu+ sensitive, Kd for Cu+ binding: 2.5x10^-18 M E.coli, yeast cells, CHO-K1 cells Copper binding domain of Amt1 CFP, YFP He C
Metabolites

glutamate

FRET glutamine reporter

FRET-based biosensors Ludewig U
Kinase Activity

Aurora B kinase

aurora B sensor

FRET-based biosensors confocal flourescence microscopy Xenopus S3, HeLa Cells, DU145, DLD21 reversible binding to an FHA2 phospho-threonine binding domain CFP, YFP Kapoor TM
Metabolites

PtdIns(3,4)P2

Pippi-PI(3,4)P2

FRET-based biosensors COS-7 cells Matsuda M
Mechanical Force

Mechanical strain

sstFRET

FRET-based biosensors confocal microscopy HEK-293, 3T3, C.Elegans Cerulean, Venus Sachs F
Neurotrophic Factor

Neurotrophic factor

BBD-ECat/ECaus (cell-based)

FRET-based biosensors CHO-EGFR cells , MCF-7 cells CFP, YFP Umezawa Y
Metabolites

glutamate

FLIP-cpGlnH183

FRET-based biosensors Ito T
Kinase Activity

CyclinB-Cdk1

CyclinB-Cdk1 sensor

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase Specific HeLa cells, Xenopus, MEF, hTert-RPE1 cells Polo box domain (PBD) of PIk1 CFP(mCerulean) , YFP(YPet) Pines J
Protein Activation and Conformation

activation of plasma membrane Ca2+ pump

BFP-PMCA-GFP

FRET-based biosensors spectroscopy concentration of BFP-PMCA-GFP-26 nM, PMCA-GFP-30 nM Saccharomyces cerevisiae strain DBY 2062, yeast cells Ca2+-calmodulin binding BFP, GFP Adamo HP
Metabolites

histidine

FLIP-HisJ/cpHisJ194

FRET-based biosensors Ito T
Kinase Activity

epidermal growth factor receptor (EGFR)

EGFR reporter

FRET-based biosensors HeLa cells, NIH 3T3 CFP, YFP Tsien RY
Protein Activation and Conformation

Akt conformation

ReAktion

FRET-based biosensors fluorescence microscopy NIH3T3 fibroblasts human Akt-1/protein kinase B-a eCFP, Citrine Zhang J
Protein Activation and Conformation

Akt conformation

GFP–Akt–YFP

FRET-based biosensors FLIM NIH 3T3 protein kinase B (PKB)/Akt eGFP , eYFP Larijani B
Metabolites

inositol 1,4,5-trisphosphate

LIBRA

FRET-based biosensors Mikoshiba K
Redox and Voltage

voltage

VSFP-cpEGFP

FRET-based biosensors Sakai R, Isacoff EY
Kinase Activity

epidermal growth factor receptor (EGFR)

ECaus

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase Specific CHO-EGFR cells Substrate, SH2 domain CFP, YFP Umezawa Y
Metabolites

arabinose

FLIParaF.Ec-250n

FRET-based biosensors spectroscopy Kd-230nM ± 18 nM E. coli BL21-Gold(DE3) E.coli K12 araF eCFP, Venus Frommer WB Available
Protein Activation and Conformation

Annexin A4 self-association

CYNEX4

FRET-based biosensors confocal flourescence microscopy, FRAP HeLa cells, N1E-115, MDCK2 neuroblastoma cells Annexin A4 (Ca2+ regulated phospholated) eCFP, eYFP Schultz C
Metabolites

inositol 1,4,5-trisphosphate

FIRE1-3

FRET-based biosensors Mignery GA

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