Filters
Sensor Target Typesort ascending Specificity Sensor Name Sensor Type Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs
Protein Activation and Conformation

Akt conformation

GFP–Akt–YFP

FRET-based biosensors FLIM NIH 3T3 protein kinase B (PKB)/Akt eGFP , eYFP Larijani B
Protein Activation and Conformation

Annexin A4 self-association

CYNEX4

FRET-based biosensors confocal flourescence microscopy, FRAP HeLa cells, N1E-115, MDCK2 neuroblastoma cells Annexin A4 (Ca2+ regulated phospholated) eCFP, eYFP Schultz C
Protein Activation and Conformation

CaMKII conformation

CamuiαamCYCaMIIα

FRET-based biosensors two-photon fluorescence microscopy HeLa cells YFP(Venus) , CFP (S175G) Schultz C, Hayashi Y
Protein Activation and Conformation

ERK conformation

Miu2

FRET-based biosensors HeLa cells, COS-7 cells, 293F CFP, YFP, RFP(Dropna) Matsuda M
Protein Activation and Conformation

Estrogen receptor ligand binding domain conformation

CEY

FRET-based biosensors COS-7 cells, Sf9 cells, Hf7c cells ER-Ligand binding domain seCFP, eYFP Lannigan DA
Protein Activation and Conformation

G protein activation

FP-Gα/FP-Gβ(γ)

FRET-based biosensors HeLa cells, HEK cells, HEK293T, Dictyostelium discoideum cells, FRT cells, haploid yeast cells YFP(Gaq-mYFP) and CFP(mTq), YFP and CFP, eYFP Gadella TW Jr, Lohse MJ, Bünemann M, Gilman AG, Bünemann M, Devreotes P, Scarlata S, Simon MI
Protein Activation and Conformation

Insulin receptor activation

Sinphos

Single FP-based extrinsic biosensors CHO-IR cells, CHO-K1 cells CFP(cpFP), GFP(cpFP) Umezawa Y
Protein Activation and Conformation

M(1) muscarinic receptor (M(1)R) activation

M1R-YFP-CFP

FRET-based biosensors fluorescence microscopy tsA-201 cells eCFP, eYFP Hille B
Protein Activation and Conformation

MAP kinase-activated protein kinase 2 conformation

GMB

FRET-based biosensors HEK293 cells, Swiss 3T3 cells eCFP, eBFP Gaestel M
Protein Activation and Conformation

PKB conformation

GFP-PKB-RFP

FRET-based biosensors NIH 3T3 eGFP , RFP Larijani B
Protein Activation and Conformation

Rho family GTPase activation

Raichu-Rap1

FRET-based biosensors Matsuda M, Matsuda M
Protein Activation and Conformation

Rho family GTPase activation

Raichu-Rap1

FRET-based biosensors Matsuda M, Matsuda M
Protein Activation and Conformation

Rho family GTPase activation

Raichu-Rac

FRET-based biosensors COS-1 cells, HEK293 cells, 293T cells, HT1080 cells, HeLa cells CFP and YFP Matsuda M, Matsuda M
Protein Activation and Conformation

Rho family GTPase activation

Raichu-Cdc42

FRET-based biosensors Matsuda M, Matsuda M
Protein Activation and Conformation

Rho family GTPase activation

Raichu-CRIB

FRET-based biosensors Matsuda M
Neurotrophic Factor

Neurotrophic factor

BBD-ECat/ECaus (cell-based)

FRET-based biosensors CHO-EGFR cells , MCF-7 cells CFP, YFP Umezawa Y
Metabolites

PtdIns(4)P

Pippi-PI(4)P

FRET-based biosensors MDCK cells Matsuda M
Metabolites

cGMP

Cygnets

FRET-based biosensors confocal flourescence microscopy cGMP Kd - 600 nM, cAMP Kd - 300 µM rat aortic smooth muscle cells, Sf9 insect cells, RFL-6 fibroblast cells PKG-Ia binding eCFP, Citrine Dostmann WR
Metabolites

PtdIns(3,4,5)P3

GFP-PHCRAC

Translocation-based biosensors Snyder SH
Metabolites

PtdIns3P

GFP-FYVEEEA1

Translocation-based biosensors Emr SD
Metabolites

cGMP

CGYs

FRET-based biosensors confocal flourescence microscopy CHO-K1 cells, HEK-293 cells PKG-Ia binding eCFP, eYFP Umezawa Y
Metabolites

PtdIns(3,4,5)P3

GFP-Ent3p

Translocation-based biosensors Letourneur F
Metabolites

PtdIns3P

GFP–p40phox iPX

Stephens LR
Metabolites

cGMP

cGES sensors

FRET-based biosensors confocal flourescence microscopy HEK-293 cells GAF-B domain of PDE2A and GAF-A domain of PDE5A eCFP, eYFP Lohse MJ
Metabolites

PtdIns(3,4,5)P3

CAY

FRET-based biosensors Swiss 3T3 fibroblasts Allen PG

Pages