Filters
Sensor Target Type Specificity Sensor Name Sensor Typesort descending Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs Sequence
Metabolites

diacylglycerol

DAGR

FRET-based biosensors confocal flourescence microscopy MDCK cells Cysteine-rich domain (CRD) CFP, YFP Umezawa Y
Redox and Voltage

redox

CY-RL5 and CY-RL7

FRET-based biosensors CFP and eYFP, mCyPet and mYPet Gaskins HR, Gaskins HR
Kinase Activity

Protein kinase C

CKAR; KCP-1

FRET-based biosensors Schultz C, Newton AC
Metabolites

sucrose

FLIPsuc sensors

FRET-based biosensors Frommer WB
Ions

Ca2+

Cameleons

FRET-based biosensors Tsien RY, Miyawaki A, Tsien RY, Persechini A, Ikura M
Ions

Ca2+

FIP-CBSMTN-XL

FRET-based biosensors Miyawaki A, Persechini A,, Ikura M
Metabolites

diacylglycerol

CKAR

FRET-based biosensors fluorescence microscopy, spectroscopy Sensitive to calyculcin A and PKC HeLa cells, MDCK cells FHA2 domain of Rad53p mCFP, mYFP Newton AC
Redox and Voltage

redox

HSP-FRET

FRET-based biosensors CFP, YFP Schumacker PT
Kinase Activity

Abl

Crk-based reporter

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific (tyrosine) NIH 3T3, MEF cells complete chicken c-CrkII gene eCFP, eYFP (citrine) Tsien RY
Kinase Activity

Protein kinase D

DKAR

FRET-based biosensors COS-7 cells, HeLa Cells mCFP, mYFP Newton AC
Metabolites

tryptophan

FLIPW sensors

FRET-based biosensors Frommer WB
Ions

Ca2+

TN-XXL

FRET-based biosensors Griesbeck O, Miyawaki A, Persechini A, Ikura M
Metabolites

glucose

FLIPglu sensors

FRET-based biosensors confocal flourescence microscopy, spectroscopy KD 600µM Saccharomyces, arabidopsis, HEK293T, COS-7 cells mature glucose/galactose-binding protein MglB eCFP, eYFP Frommer WB, Frommer WB, Frommer WB
Redox and Voltage

redox

Redoxfluor

FRET-based biosensors CHO cells Cerulean, Citrine Sakai Y
Kinase Activity

Abl

EGFR indicator

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific, sensitive to concentration of EGF B82, NIH 3T3, HeLa cells SH2 domain (residues 374–465) from mouse p52 Shc eCFP, eYFP (citrine) Tsien RY
Kinase Activity

Src

Src reporter

FRET-based biosensors MCF-7 cells CFP, YFP Umezawa Y
Ions

Cl-

Clomeleon

FRET-based biosensors confocal flourescence microscopy PKD1 (K467A) Kd: .196 µM, WT PKD1 - .012, µM, PKD1 (K467R) - .009 µM E.coli, HEK-293 cells PDK1 PH domain green fluorescent protein (GFP-) tagged versions of wild-type PDK1 van Aalten DM
Metabolites

glucose

AcGFP1-GBPcys-mCherry

FRET-based biosensors Ye K
Kinase Activity

Abl

Src indicator

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific (tyrosine) NIH 3T3, HeLa Cells Rous sarcoma virus Src gene (residues148–245) eCFP, eYFP (citrine) Tsien RY
Kinase Activity

Src

Srcus

FRET-based biosensors HeLa cells, MEF cells CFP, YFP Chien S
Ions

Cl-

Cl-sensor

FRET-based biosensors fluorescence microscopy, spectroscopy pH-specific/sensitive CHO-K1 cells, HEK cells, hippocampal neurons, photoreceptors from rat retina peptide linker mutated eYFP, CFP Bregestovski P Plain text icon test_sequence_ bregestovski.txt
Metabolites

glutamate

FLIPE sensors

FRET-based biosensors confocal flourescence microscopy, spectroscopy KD 630 nM Rat hippocampal cells, PC12 cells glutamate/aspartate binding protein ybeJ from Escherichia coli eCFP, Venus Frommer WB, Frommer WB
Kinase Activity

Abl

Picchu

FRET-based biosensors fluorescence microscopy Kinase Specific (tyrosine) HeLa cells, Cos7/E3 Src-homology (SH) 2 of Crk II or phosphotyrosine-binding (PTB) eYFP, CFP Matsuda M
Mechanical Force

Mechanical strain

VinTS

FRET-based biosensors confocal microscopy BAECs, MEF cells, HEK293 cells mTFP1, venus(A206K) Schwartz MA
Ions

Cu+

Ace1-FRET

FRET-based biosensors fluorescence microscopy, spectroscopy Cu+ sensitive Yeast cells, E.coli Copper (I) binding domain CFP, YFP He C

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