Filters
Sensor Target Type Specificitysort ascending Sensor Name Sensor Type Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs
Ions

pH

ECFP

Single FP-based intrinsic biosensors Tsien RY, Verkman AS
Ions

pH

EYFP

Single FP-based intrinsic biosensors Tsien RY, Verkman AS
Ions

pH

E2GFP

Single FP-based intrinsic biosensors Beltram F
Ions

pH

deGFP1-4

Single FP-based intrinsic biosensors Remington SJ
Ions

pH

pHluorins

Single FP-based intrinsic biosensors Rothman JE
Ions

pH

GFpH

Single FP-based intrinsic biosensors Miyazaki S
Ions

pH

YFpH

Single FP-based intrinsic biosensors Miyazaki S
Ions

pH

mtAlpHi

Single FP-based intrinsic biosensors Pozzan T
Ions

pH

pHRed

Single FP-based intrinsic biosensors Yellen G
Ions

pH

CFP-YFP tandem

FRET-based biosensors Schaefer M
Ions

pH

pHuji

Single FP-based intrinsic biosensors mApple Campbell RE
Enzyme Activity (Non-Kinase)

O-GlcNAc transferase

O-GlcNAc sensor

FRET-based biosensors HeLa cells eCFP, Venus Mahal LK
Metabolites

NO

FRET-MT

FRET-based biosensors Levitan ES
Neurotrophic Factor

Neurotrophic factor

BBD-ECat/ECaus (cell-based)

FRET-based biosensors CHO-EGFR cells , MCF-7 cells CFP, YFP Umezawa Y
Metabolites

NADP+

Apollo-NADP++

Steady-state fluorescence anisotropy due to FRET Cerulean Rocheleau JV
Metabolites

NADH

Peredox

Single FP-based extrinsic biosensors cpT-Sapphire Yellen G
Metabolites

NAD+

none

Single FP-based extrinsic biosensors venus Goodman RH
Kinase Activity

MK2

GFP-MK2-BFP

FRET-based biosensors HEK-293, Swiss 3T3 cells eGFP , eBFP Gaestel M
Mechanical Force

Mechanical strain

VinTS

FRET-based biosensors confocal microscopy BAECs, MEF cells, HEK293 cells mTFP1, venus(A206K) Schwartz MA
Mechanical Force

Mechanical strain

stFRET

FRET-based biosensors Dictyostelium , HS1, AX2 GFP, eGFP Uyeda TQ
Mechanical Force

Mechanical strain

PriSSM (PRIM-based)

FRET-based biosensors BAECs, HEK cells Cerulean, Venus Sachs F
Mechanical Force

Mechanical strain

sstFRET

FRET-based biosensors confocal microscopy HEK-293, 3T3, C.Elegans Cerulean, Venus Sachs F
Protein Activation and Conformation

MAP kinase-activated protein kinase 2 conformation

GMB

FRET-based biosensors HEK293 cells, Swiss 3T3 cells eCFP, eBFP Gaestel M
Metabolites

maltose

FLIPmal sensors

FRET-based biosensors confocal flourescence microscopy arabinose FLIP Kd-200 nM,maltose FLIP Kd-40 µM Escherichia coli BL21-Gold(DE3) cells periplasmic binding protein for arabinose or maltose, E. coli high-affinity L-arabinose binding protein AraF eCFP, eYFP (venus) Frommer WB
Protein Activation and Conformation

M(1) muscarinic receptor (M(1)R) activation

M1R-YFP-CFP

FRET-based biosensors fluorescence microscopy tsA-201 cells eCFP, eYFP Hille B

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