Filters
Sensor Target Type Specificity Sensor Name Sensor Typesort descending Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs Addgene Link
Enzyme Activity (Non-Kinase)

histone lysine methylation

K9 reporter

FRET-based biosensors MEF cells CFP, YFP Ting AY
Metabolites

cAMP

PKA-camps

FRET-based biosensors spectroscopy, fluorescence microscopy sensitive cAMP indicators Sf9 cells, CHO-A2B, TsA201, HEK293, COS-7 cells cAMP binding eCFP, eYFP Lohse MJ
Protein Activation and Conformation

Rho family GTPase activation

Raichu-Rap1

FRET-based biosensors Matsuda M, Matsuda M
Ions

pH

CFP-YFP tandem

FRET-based biosensors Schaefer M
Kinase Activity

Insulin Receptor

Phocus-2pp

FRET-based biosensors CHO-IR cells eCFP, eYFP Umezawa Y
Enzyme Activity (Non-Kinase)

histone lysine methylation

K27 reporter

FRET-based biosensors Ting AY
Metabolites

cAMP

HCN2-camps

FRET-based biosensors confocal flourescence microscopy, FRAP cAMP-sensitive HEK 293a cells, mouse heart single cAMP binding of HCN2 channel- a helices covering cAMP binding site eCFP, eYFP Engelhardt S
Protein Activation and Conformation

Rho family GTPase activation

Raichu-Rac

FRET-based biosensors COS-1 cells, HEK293 cells, 293T cells, HT1080 cells, HeLa cells CFP and YFP Matsuda M, Matsuda M
Ions

PO42-

FLIPPi

FRET-based biosensors confocal flourescence microscopy Kd - 30mM COS-7 cells, CHO cells, E. coli BL21(DE3)Gold cells Synechococcus phosphate-binding protein (PiBP) eCFP, Venus Frommer WB Available
Kinase Activity

MK2

GFP-MK2-BFP

FRET-based biosensors HEK-293, Swiss 3T3 cells eGFP , eBFP Gaestel M
Enzyme Activity (Non-Kinase)

O-GlcNAc transferase

O-GlcNAc sensor

FRET-based biosensors HeLa cells eCFP, Venus Mahal LK
Metabolites

cAMP

(T)Epac(VV)

FRET-based biosensors FLIM, confocal fluorescence microscopy cAMP indicators Hek293 cells, N1E-115, human HeLa cells (ccl-2) cAMP binding mTurquise (mECFPD-Epac(CD, and DEP)-cp173Venus-Venus Jalink K
Protein Activation and Conformation

Rho family GTPase activation

Raichu-Cdc42

FRET-based biosensors Matsuda M, Matsuda M
Ions

Zn2+

CLY9-2His

FRET-based biosensors Merkx M
Kinase Activity

Protein kinase A

ART

FRET-based biosensors COS-7 cells BFP, RFP Hagiwara, M
Enzyme Activity (Non-Kinase)

protein phosphatase (calcineurin)

CaNAR1

FRET-based biosensors HeLa cells eCFP, cpV(L194)(Venus) Zhang J
Protein Activation and Conformation

Rho family GTPase activation

Raichu-CRIB

FRET-based biosensors Matsuda M
Ions

Zn2+

CALWY

FRET-based biosensors Merkx M
Kinase Activity

Protein kinase A

AKAR1-34

FRET-based biosensors HEK293 cells Various Tsien RY, Zhang J, Zhang J
Enzyme Activity (Non-Kinase)

various proteases

donor FP-substrate-acceptor FP

FRET-based biosensors HeLa cells eCFP, eBFP Luo Y
Metabolites

cGMP

Cygnets

FRET-based biosensors confocal flourescence microscopy cGMP Kd - 600 nM, cAMP Kd - 300 µM rat aortic smooth muscle cells, Sf9 insect cells, RFL-6 fibroblast cells PKG-Ia binding eCFP, Citrine Dostmann WR
Protein Activation and Conformation

Rho family GTPase activation

Raichu-RhoA

FRET-based biosensors Matsuda M
Ions

Zn2+

eCALWYs

FRET-based biosensors Pancreatic beta-cells Rutter GA, Merkx M
Kinase Activity

Protein kinase A

CRY-AKAR

FRET-based biosensors HeLa cells Cerulean, mVenus, mCherry Zhang J
Metabolites

PtdIns(4)P

Pippi-PI(4)P

FRET-based biosensors MDCK cells Matsuda M

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