Filters
Sensor Target Type Specificity Sensor Name Sensor Typesort descending Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs Addgene Link
Metabolites

PtdIns3P

GFP–p40phox iPX

Stephens LR
Enzyme Activity (Non-Kinase)

histone H4 lysine acetylation

Histac (K5, K7)

FRET-based biosensors CFP, YFP(venus) Yoshida M
Kinase Activity

Extracellular signal-regulated kinase (ERK)

Miu2

FRET-based biosensors HeLa cells CFP, YFP Matsuda M
Metabolites

cAMP

FP-PKARII/FP-PKAcat

FRET-based biosensors eBFP and GFP or CFP and YFP Pozzan T, Pozzan T
Protein Activation and Conformation

M(1) muscarinic receptor (M(1)R) activation

M1R-YFP-CFP

FRET-based biosensors fluorescence microscopy tsA-201 cells eCFP, eYFP Hille B
Kinase Activity

Glucokinase

mCerulean-GCK-mVenus

FRET-based biosensors confocal flourescence microscopy BTC3 cells YFP Rizzo MA
Metabolites

cAMP

CFP–Epac–YFP

FRET-based biosensors fluorescence microscopy highly sensitive cAMP indicator, Kd of 2.3 µM human A431 cells, Rat-1 and NIH3T3 fibroblasts, mouse GE11 epithelial cells, mouse N1E-115 neuroblastoma, human MCF7 breast carcinoma cells cAMP binding CFP, YFP Jalink K
Protein Activation and Conformation

MAP kinase-activated protein kinase 2 conformation

GMB

FRET-based biosensors HEK293 cells, Swiss 3T3 cells eCFP, eBFP Gaestel M
Kinase Activity

Histone H3 phosphorylation

H3 phosphorylation reporter

FRET-based biosensors inverted epiflourescence microscopy HeLa cells CFP, YFP Ting AY
Metabolites

PtdIns(4,5)P2

CAY

FRET-based biosensors Swiss 3T3 fibroblasts Allen PG
Enzyme Activity (Non-Kinase)

histone H4 lysine acetylation

Histac-K12 (K12)

FRET-based biosensors confocal microscopy COS7 cells CFP, YFP(venus) Yoshida M
Metabolites

cAMP

ICUEs

FRET-based biosensors eCFP, Citrine Zhang J, Zhang J, Lefkowitz RJ
Protein Activation and Conformation

PKB conformation

GFP-PKB-RFP

FRET-based biosensors NIH 3T3 eGFP , RFP Larijani B
Kinase Activity

Insulin Receptor

Phocus

FRET-based biosensors Immunofluorescence microscopy CHO-IR cells eCFP, eYFP Umezawa Y
FRET-based biosensors

PtdIns(4,5)P2

Pippi-PI(4,5)P2

FRET-based biosensors MDCK cells Matsuda M
Enzyme Activity (Non-Kinase)

histone lysine methylation

K9 reporter

FRET-based biosensors MEF cells CFP, YFP Ting AY
Metabolites

cAMP

Epac-camps

FRET-based biosensors spectroscopy, fluorescence microscopy sensitive cAMP indicators Sf9 cells, CHO-A2B, TsA201, HEK293, COS-7 cells cAMP binding eCFP, eYFP Lohse MJ
Protein Activation and Conformation

Rho family GTPase activation

Raichu-Rap1

FRET-based biosensors Matsuda M, Matsuda M
Ions

pH

CFP-YFP tandem

FRET-based biosensors Schaefer M
Kinase Activity

Insulin Receptor

Phocus-2pp

FRET-based biosensors CHO-IR cells eCFP, eYFP Umezawa Y
Enzyme Activity (Non-Kinase)

histone lysine methylation

K27 reporter

FRET-based biosensors Ting AY
Metabolites

cAMP

PKA-camps

FRET-based biosensors spectroscopy, fluorescence microscopy sensitive cAMP indicators Sf9 cells, CHO-A2B, TsA201, HEK293, COS-7 cells cAMP binding eCFP, eYFP Lohse MJ
Protein Activation and Conformation

Rho family GTPase activation

Raichu-Rap1

FRET-based biosensors Matsuda M, Matsuda M
Ions

PO42-

FLIPPi

FRET-based biosensors confocal flourescence microscopy Kd - 30mM COS-7 cells, CHO cells, E. coli BL21(DE3)Gold cells Synechococcus phosphate-binding protein (PiBP) eCFP, Venus Frommer WB Available
Kinase Activity

MK2

GFP-MK2-BFP

FRET-based biosensors HEK-293, Swiss 3T3 cells eGFP , eBFP Gaestel M

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