Filters
Sensor Target Typesort ascending Specificity Sensor Name Sensor Type Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs
Kinase Activity

Protein kinase B/Akt

Akind

FRET-based biosensors COS-7 cells CFP, YFP Matsuda M
Kinase Activity

Protein kinase B/Akt

GFP-PKB-RFP

FRET-based biosensors NIH 3T3 GFP, RFP Larijani B
Kinase Activity

Protein kinase B/Akt

ReAktion

FRET-based biosensors NIH 3T3 CFP, YFP Zhang J
Kinase Activity

Protein kinase B/Akt

AktAR

FRET-based biosensors NIH 3T3 Cerulean, cpV E172 Zhang J
Kinase Activity

Protein kinase C

CKAR; KCP-1

FRET-based biosensors Schultz C, Newton AC
Kinase Activity

Abl

Crk-based reporter

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific (tyrosine) NIH 3T3, MEF cells complete chicken c-CrkII gene eCFP, eYFP (citrine) Tsien RY
Kinase Activity

Protein kinase D

DKAR

FRET-based biosensors COS-7 cells, HeLa Cells mCFP, mYFP Newton AC
Kinase Activity

Abl

EGFR indicator

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific, sensitive to concentration of EGF B82, NIH 3T3, HeLa cells SH2 domain (residues 374–465) from mouse p52 Shc eCFP, eYFP (citrine) Tsien RY
Kinase Activity

Src

Src reporter

FRET-based biosensors MCF-7 cells CFP, YFP Umezawa Y
Kinase Activity

Abl

Src indicator

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific (tyrosine) NIH 3T3, HeLa Cells Rous sarcoma virus Src gene (residues148–245) eCFP, eYFP (citrine) Tsien RY
Kinase Activity

Src

Srcus

FRET-based biosensors HeLa cells, MEF cells CFP, YFP Chien S
Kinase Activity

Abl

Picchu

FRET-based biosensors fluorescence microscopy Kinase Specific (tyrosine) HeLa cells, Cos7/E3 Src-homology (SH) 2 of Crk II or phosphotyrosine-binding (PTB) eYFP, CFP Matsuda M
Kinase Activity

Abl

Picchu-Z/EGFR-Z

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific (tyrosine) Cos1 and HT1080 and NIH3T3 and A431 Src-homology (SH) 2 and SH3 from Crk II YFP, CFP Matsuda M
Kinase Activity

ATM-Protein Kinase

ATOMIC

FRET-based biosensors confocal flourescence microscopy irreversible HeLa cells, NIH 3T3, AT4Bi Rad53 FHA2 (Chk2) and peptide with T68 phosphorylation site CFP, YFP Hunter T
Kinase Activity

Aurora B kinase

aurora B sensor

FRET-based biosensors confocal flourescence microscopy Xenopus S3, HeLa Cells, DU145, DLD21 reversible binding to an FHA2 phospho-threonine binding domain CFP, YFP Kapoor TM
Kinase Activity

CyclinB-Cdk1

CyclinB-Cdk1 sensor

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase Specific HeLa cells, Xenopus, MEF, hTert-RPE1 cells Polo box domain (PBD) of PIk1 CFP(mCerulean) , YFP(YPet) Pines J
Kinase Activity

epidermal growth factor receptor (EGFR)

EGFR reporter

FRET-based biosensors HeLa cells, NIH 3T3 CFP, YFP Tsien RY
Kinase Activity

epidermal growth factor receptor (EGFR)

ECaus

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase Specific CHO-EGFR cells Substrate, SH2 domain CFP, YFP Umezawa Y
Kinase Activity

epidermal growth factor receptor (EGFR)

Bescell/ BBD-Ecat

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase specific, selective to BDNF MCF-7 cells BDNF binding to BBD-Ecat domain CFP, YFP
Kinase Activity

epidermal growth factor receptor (EGFR)

EGFR-ECFP/PTV-EYFP

FRET-based biosensors FLIM, fluorescence microscopy PTB domain (KD, 140 nM to 5.3 µM) MCF-7 cells Phosphotyrosine (PTB) domain of human Shc eCFP, eYFP (citrine) Bastiaens PI
Kinase Activity

epidermal growth factor receptor (EGFR)

EGFR-ECFP/PTV-EYFP

FRET-based biosensors FLIM, fluorescence microscopy PTB domain (KD, 140 nM to 5.3 µM) COS-7 cells PTB domain eCFP, eYFP Bastiaens PI
Kinase Activity

Extracellular signal-regulated kinase (ERK)

Erkus

FRET-based biosensors confocal flourescence microscopy Sensitive to ERK activity MCF-7 cells FHA2 domain and ERK docking domain eCFP, eYFP Umezawa Y
Kinase Activity

Extracellular signal-regulated kinase (ERK)

EAS

FRET-based biosensors spectroscopy Sensitive to ERK activity BL21(DE3) cells, NIH 3T3 Serine-Proline (SP) binding, either D-domain or DEF domain eCFP, eYFP Alberola-Ila J
Kinase Activity

Extracellular signal-regulated kinase (ERK)

EKAR

FRET-based biosensors FLIM, fluorescence microscopy Sensitive to p38 and JNK and ERK activities HEK293 cells, pyramidal neurons, COS-7 cells Phospho-binding domain and ERK docking domain eGFP , mRFP1 or CFP , YFP Svoboda K
Ions

pH

GFpH

Single FP-based intrinsic biosensors Miyazaki S

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