Filters
Sensor Target Typesort ascending Specificity Sensor Name Sensor Type Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs
Kinase Activity

epidermal growth factor receptor (EGFR)

EGFR reporter

FRET-based biosensors HeLa cells, NIH 3T3 CFP, YFP Tsien RY
Kinase Activity

epidermal growth factor receptor (EGFR)

ECaus

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase Specific CHO-EGFR cells Substrate, SH2 domain CFP, YFP Umezawa Y
Kinase Activity

epidermal growth factor receptor (EGFR)

Bescell/ BBD-Ecat

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase specific, selective to BDNF MCF-7 cells BDNF binding to BBD-Ecat domain CFP, YFP
Kinase Activity

epidermal growth factor receptor (EGFR)

EGFR-ECFP/PTV-EYFP

FRET-based biosensors FLIM, fluorescence microscopy PTB domain (KD, 140 nM to 5.3 µM) MCF-7 cells Phosphotyrosine (PTB) domain of human Shc eCFP, eYFP (citrine) Bastiaens PI
Kinase Activity

epidermal growth factor receptor (EGFR)

EGFR-ECFP/PTV-EYFP

FRET-based biosensors FLIM, fluorescence microscopy PTB domain (KD, 140 nM to 5.3 µM) COS-7 cells PTB domain eCFP, eYFP Bastiaens PI
Kinase Activity

Extracellular signal-regulated kinase (ERK)

Erkus

FRET-based biosensors confocal flourescence microscopy Sensitive to ERK activity MCF-7 cells FHA2 domain and ERK docking domain eCFP, eYFP Umezawa Y
Kinase Activity

Extracellular signal-regulated kinase (ERK)

EAS

FRET-based biosensors spectroscopy Sensitive to ERK activity BL21(DE3) cells, NIH 3T3 Serine-Proline (SP) binding, either D-domain or DEF domain eCFP, eYFP Alberola-Ila J
Kinase Activity

Extracellular signal-regulated kinase (ERK)

EKAR

FRET-based biosensors FLIM, fluorescence microscopy Sensitive to p38 and JNK and ERK activities HEK293 cells, pyramidal neurons, COS-7 cells Phospho-binding domain and ERK docking domain eGFP , mRFP1 or CFP , YFP Svoboda K
Kinase Activity

Extracellular signal-regulated kinase (ERK)

Miu2

FRET-based biosensors HeLa cells CFP, YFP Matsuda M
Kinase Activity

Glucokinase

mCerulean-GCK-mVenus

FRET-based biosensors confocal flourescence microscopy BTC3 cells YFP Rizzo MA
Kinase Activity

Histone H3 phosphorylation

H3 phosphorylation reporter

FRET-based biosensors inverted epiflourescence microscopy HeLa cells CFP, YFP Ting AY
Kinase Activity

Insulin Receptor

Phocus

FRET-based biosensors Immunofluorescence microscopy CHO-IR cells eCFP, eYFP Umezawa Y
Kinase Activity

Insulin Receptor

Phocus-2pp

FRET-based biosensors CHO-IR cells eCFP, eYFP Umezawa Y
Kinase Activity

MK2

GFP-MK2-BFP

FRET-based biosensors HEK-293, Swiss 3T3 cells eGFP , eBFP Gaestel M
Kinase Activity

Protein kinase A

ART

FRET-based biosensors COS-7 cells BFP, RFP Hagiwara, M
Kinase Activity

Protein kinase A

AKAR1-34

FRET-based biosensors HEK293 cells Various Tsien RY, Zhang J, Zhang J
Kinase Activity

Protein kinase A

CRY-AKAR

FRET-based biosensors HeLa cells Cerulean, mVenus, mCherry Zhang J
Kinase Activity

Protein kinase B/Akt

BKAR

FRET-based biosensors NIH 3T3 mCFP, mYFP Newton AC
Kinase Activity

Protein kinase B/Akt

Aktus

FRET-based biosensors CHO cells CFP, YFP Umezawa Y
Kinase Activity

Protein kinase B/Akt

Akind

FRET-based biosensors COS-7 cells CFP, YFP Matsuda M
Kinase Activity

Protein kinase B/Akt

GFP-PKB-RFP

FRET-based biosensors NIH 3T3 GFP, RFP Larijani B
Kinase Activity

Protein kinase B/Akt

ReAktion

FRET-based biosensors NIH 3T3 CFP, YFP Zhang J
Kinase Activity

Protein kinase B/Akt

AktAR

FRET-based biosensors NIH 3T3 Cerulean, cpV E172 Zhang J
Kinase Activity

Protein kinase C

CKAR; KCP-1

FRET-based biosensors Schultz C, Newton AC
Ions

Ca2+

FIP-CBSMTN-XL

FRET-based biosensors Miyawaki A, Persechini A,, Ikura M

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