Filters
Sensor Target Typesort ascending Specificity Sensor Name Sensor Type Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs
Metabolites

PtdIns(3,5)P2

GFP-Ent3p

Translocation-based biosensors Letourneur F
Metabolites

ATP:ADP ratio

Perceval

Single FP-based extrinsic biosensors Yellen G
Metabolites

maltose

FLIPmal sensors

FRET-based biosensors confocal flourescence microscopy arabinose FLIP Kd-200 nM,maltose FLIP Kd-40 µM Escherichia coli BL21-Gold(DE3) cells periplasmic binding protein for arabinose or maltose, E. coli high-affinity L-arabinose binding protein AraF eCFP, eYFP (venus) Frommer WB
Metabolites

PtdIns(4,5)P2

GFP-ARNO

Translocation-based biosensors Czech MP
Metabolites

BAI2 (a furanosyl borate diester)

CLPY

FRET-based biosensors fluorescence microscopy 98 kDa (CLPY), BAI2 Kd 270 nM BL21 (luxS-) cells transformed with pQE30-CLPY or LuxP-mutant constructs LuxP-BAI2 binding CFP, YFP Sayre R
Metabolites

NO

FRET-MT

FRET-based biosensors Levitan ES
Metabolites

PtdIns(4,5)P2

GFP-Cytohesin-1

Translocation-based biosensors Czech MP
Metabolites

BAI2 (a furanosyl borate diester)

LuxP-FRET

FRET-based biosensors spectroscopy, fluorescence microscopy BAI2 Kd 270 nM Escherichia coli BL21 (luxS-), V. harVeyi strains BB120 (wild-type), MM30 (luxS-), MM32 (luxS-, luxN-) LuxP-BAI2 binding CFP, YFP Sayre R
Metabolites

Phosphatidic acid

Pii

FRET-based biosensors Kiyokawa E
Metabolites

PtdIns(4,5)P2

GFP-PHOSH2

Translocation-based biosensors confocal microscopy HeLa cells, yeast cells eGFP Lemmon MA
Mechanical Force

Mechanical strain

VinTS

FRET-based biosensors confocal microscopy BAECs, MEF cells, HEK293 cells mTFP1, venus(A206K) Schwartz MA
Mechanical Force

Mechanical strain

stFRET

FRET-based biosensors Dictyostelium , HS1, AX2 GFP, eGFP Uyeda TQ
Mechanical Force

Mechanical strain

PriSSM (PRIM-based)

FRET-based biosensors BAECs, HEK cells Cerulean, Venus Sachs F
Mechanical Force

Mechanical strain

sstFRET

FRET-based biosensors confocal microscopy HEK-293, 3T3, C.Elegans Cerulean, Venus Sachs F
Kinase Activity

Extracellular signal-regulated kinase (ERK)

Miu2

FRET-based biosensors HeLa cells CFP, YFP Matsuda M
Kinase Activity

Glucokinase

mCerulean-GCK-mVenus

FRET-based biosensors confocal flourescence microscopy BTC3 cells YFP Rizzo MA
Kinase Activity

Histone H3 phosphorylation

H3 phosphorylation reporter

FRET-based biosensors inverted epiflourescence microscopy HeLa cells CFP, YFP Ting AY
Kinase Activity

Insulin Receptor

Phocus

FRET-based biosensors Immunofluorescence microscopy CHO-IR cells eCFP, eYFP Umezawa Y
Kinase Activity

Insulin Receptor

Phocus-2pp

FRET-based biosensors CHO-IR cells eCFP, eYFP Umezawa Y
Kinase Activity

MK2

GFP-MK2-BFP

FRET-based biosensors HEK-293, Swiss 3T3 cells eGFP , eBFP Gaestel M
Kinase Activity

Protein kinase A

ART

FRET-based biosensors COS-7 cells BFP, RFP Hagiwara, M
Kinase Activity

Protein kinase A

AKAR1-34

FRET-based biosensors HEK293 cells Various Tsien RY, Zhang J, Zhang J
Kinase Activity

Protein kinase A

CRY-AKAR

FRET-based biosensors HeLa cells Cerulean, mVenus, mCherry Zhang J
Kinase Activity

Protein kinase B/Akt

BKAR

FRET-based biosensors NIH 3T3 mCFP, mYFP Newton AC
Kinase Activity

Protein kinase B/Akt

Aktus

FRET-based biosensors CHO cells CFP, YFP Umezawa Y

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