Filters
Sensor Target Typesort ascending Specificity Sensor Name Sensor Type Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs
Metabolites

cAMP

PKA-camps

FRET-based biosensors spectroscopy, fluorescence microscopy sensitive cAMP indicators Sf9 cells, CHO-A2B, TsA201, HEK293, COS-7 cells cAMP binding eCFP, eYFP Lohse MJ
Metabolites

PtdIns(3,4,5)P3

GFP-PHPDK1

Translocation-based biosensors Alessi DR, van Aalten DM
Metabolites

PtdIns(3,4,5)P3

YFP-PHTAPP1

Translocation-based biosensors Alessi DR
Metabolites

PtdIns(4,5)P2:Ins(1,4,5) P3 dynamics

CYPHR (FRET: PLC activity)

Translocation-based biosensors Shapiro L
Metabolites

cAMP

HCN2-camps

FRET-based biosensors confocal flourescence microscopy, FRAP cAMP-sensitive HEK 293a cells, mouse heart single cAMP binding of HCN2 channel- a helices covering cAMP binding site eCFP, eYFP Engelhardt S
Metabolites

PtdIns(3,4,5)P3

GFP-Svp1p

Translocation-based biosensors Lemmon MA
Metabolites

PtdIns(4,5)P2:Ins(1,4,5) P3 dynamics

FP-Tubby

Translocation-based biosensors Meyer T
Metabolites

cAMP

(T)Epac(VV)

FRET-based biosensors FLIM, confocal fluorescence microscopy cAMP indicators Hek293 cells, N1E-115, human HeLa cells (ccl-2) cAMP binding mTurquise (mECFPD-Epac(CD, and DEP)-cp173Venus-Venus Jalink K
Metabolites

PtdIns(3,4,5)P3

GFP-PHBtk

Translocation-based biosensors Balla T
Metabolites

PtdIns(4,5)P2:Ins(1,4,5) P3 dynamics

FP-PHPLCδ1(FRET)

Translocation-based biosensors Meyer T
Metabolites

cGMP

FlincG

Single FP-based extrinsic biosensors Dostmann WR
Metabolites

PtdIns(3,4,5)P3

GFP-PHCRAC

Translocation-based biosensors Snyder SH
Metabolites

PtdIns(4)P

Pippi-PI(4)P

FRET-based biosensors MDCK cells Matsuda M
Metabolites

cGMP

Cygnets

FRET-based biosensors confocal flourescence microscopy cGMP Kd - 600 nM, cAMP Kd - 300 µM rat aortic smooth muscle cells, Sf9 insect cells, RFL-6 fibroblast cells PKG-Ia binding eCFP, Citrine Dostmann WR
Metabolites

PtdIns(3,4,5)P3

GFP-Ent3p

Translocation-based biosensors Letourneur F
Metabolites

PtdIns3P

GFP-FYVEEEA1

Translocation-based biosensors Emr SD
Metabolites

cGMP

CGYs

FRET-based biosensors confocal flourescence microscopy CHO-K1 cells, HEK-293 cells PKG-Ia binding eCFP, eYFP Umezawa Y
Metabolites

PtdIns(3,4,5)P3

CAY

FRET-based biosensors Swiss 3T3 fibroblasts Allen PG
Metabolites

PtdIns3P

GFP–p40phox iPX

Stephens LR
Metabolites

cGMP

cGES sensors

FRET-based biosensors confocal flourescence microscopy HEK-293 cells GAF-B domain of PDE2A and GAF-A domain of PDE5A eCFP, eYFP Lohse MJ
Metabolites

PtdIns(3,4,5)P3

Pippi-PI(3,4,5)P3

FRET-based biosensors MDCK cells Matsuda M
Metabolites

PtdIns4P

GFP-PHOSH2

Translocation-based biosensors confocal microscopy Levine TP
Metabolites

cGMP

cGi sensors

FRET-based biosensors spectroscopy, fluorescence microscopy cGMP concentration 1-2 µM HEK293 cells cGMP-binding sites cNMP-BD (cGKI) or GAF (PDE5) eCFP, eYFP Koesling D
Metabolites

PtdIns(3,4,5)P3

Fllip

FRET-based biosensors CHO cells Umezawa Y
Metabolites

PtdIns4P

PHOSBP

Translocation-based biosensors confocal microscopy COS-7 cells GFP, mRFP Balla T, Munro S

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