Filters
Sensor Target Typesort ascending Specificity Sensor Name Sensor Type Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs Addgene Link
Metabolites

PtdIns4P

GFP-PHOSH2

Translocation-based biosensors confocal microscopy Levine TP
Metabolites

cGMP

Cygnus

FRET-based biosensors FLIM, confocal fluorescence microscopy, spectroscopy pH-sensitive PC12 cells, HEK 293T cells, HeLa cells cGMP binding from PDE5 mTagBFP, sREACh(dark) Oka K
Metabolites

PtdIns(3,4,5)P3

Fllip

FRET-based biosensors CHO cells Umezawa Y
Metabolites

PtdIns4P

PHOSBP

Translocation-based biosensors confocal microscopy COS-7 cells GFP, mRFP Balla T, Munro S
Metabolites

citrate

CIT sensors

FRET-based biosensors fluorescence microscopy Kd range - 8 µM - 1765 µM Kd: CFP-CitA6-130-Venus - 8 µM COS cells, arabidopsis, yeast, e.coli citrate binding of the Klebsiella pneumoniae histidine sensor kinase CitA venus, CFP Zamboni N
Metabolites

PtdIns(3,4,5)P3

Pippi-PI(3,4,5)P3

FRET-based biosensors COS-7 cells Matsuda M
Metabolites

PtdIns4P

GFP-PHFAPP

Translocation-based biosensors confocal microscopy HEK-293 cells GFP Balla T, De Matteis MA
Metabolites

diacylglycerol

Daglas

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase Specific MDCK cells, NIH 3T3 C1 domain of PKCßII as DAG binding domain CFP, YFP Matsuda M
Metabolites

PtdIns(3,4)P2

GFP-PHGRP1

Translocation-based biosensors confocal microscopy PC12 cells GFP Cullen PJ
Metabolites

ribose

FLIPrib sensors

FRET-based biosensors Lalonde S
Metabolites

diacylglycerol

DAGR

FRET-based biosensors confocal flourescence microscopy MDCK cells Cysteine-rich domain (CRD) CFP, YFP Umezawa Y
Metabolites

PtdIns(3,4)P2

GFP-PHPDK1

Translocation-based biosensors Alessi DR, van Aalten DM
Metabolites

sucrose

FLIPsuc sensors

FRET-based biosensors Frommer WB
Metabolites

diacylglycerol

CKAR

FRET-based biosensors fluorescence microscopy, spectroscopy Sensitive to calyculcin A and PKC HeLa cells, MDCK cells FHA2 domain of Rad53p mCFP, mYFP Newton AC
Metabolites

PtdIns(3,4)P2

YFP-PHTAPP1

Translocation-based biosensors Alessi DR
Metabolites

tryptophan

FLIPW sensors

FRET-based biosensors Frommer WB
Metabolites

glucose

FLIPglu sensors

FRET-based biosensors confocal flourescence microscopy, spectroscopy KD 600µM Saccharomyces, arabidopsis, HEK293T, COS-7 cells mature glucose/galactose-binding protein MglB eCFP, eYFP Frommer WB, Frommer WB, Frommer WB
Metabolites

PtdIns(3,4)P2

GFP-Svp1p

Translocation-based biosensors Lemmon MA
Metabolites FRET-Based sensor confocal microscopy Kd 1: 8 uM/ Kd 2: 800 uM HeLa cells, Astrocytes, HEK293 cells, hippocampal neurons LldR mTFP, Venus Felipe Barros Available
Metabolites

glucose

AcGFP1-GBPcys-mCherry

FRET-based biosensors Ye K
Metabolites

PtdIns(3,4)P2

GFP-PHBtk

Translocation-based biosensors Balla T
Metabolites FRET-Based sensor confocal microscopy Kd: 107 uM HeLa cells, Astrocytes, HEK293 cells, hippocampal neurons PdhR mTFP, Venus Felipe Barros Available
Metabolites

glutamate

FLIPE sensors

FRET-based biosensors confocal flourescence microscopy, spectroscopy KD 630 nM Rat hippocampal cells, PC12 cells glutamate/aspartate binding protein ybeJ from Escherichia coli eCFP, Venus Frommer WB, Frommer WB
Metabolites

PtdIns(3,4)P2

GFP-Ent3p

Translocation-based biosensors Letourneur F
Metabolites

NAD+

none

Single FP-based extrinsic biosensors venus Goodman RH

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