Filters
Sensor Target Typesort ascending Specificity Sensor Name Sensor Type Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs Addgene Link
Metabolites

NAD+

none

Single FP-based extrinsic biosensors venus Goodman RH
Metabolites

glutamate

FLIPE sensors

FRET-based biosensors confocal flourescence microscopy, spectroscopy KD 630 nM Rat hippocampal cells, PC12 cells glutamate/aspartate binding protein ybeJ from Escherichia coli eCFP, Venus Frommer WB, Frommer WB
Metabolites

PtdIns(3,4)P2

GFP-Ent3p

Translocation-based biosensors Letourneur F
Metabolites

NADP+

Apollo-NADP++

Steady-state fluorescence anisotropy due to FRET Cerulean Rocheleau JV
Metabolites

glutamate

GluSnFR

FRET-based biosensors Tsien RY
Metabolites

PtdIns(3,4)P2

CAY

FRET-based biosensors Swiss 3T3 fibroblasts Allen PG
Metabolites

NADH

Peredox

Single FP-based extrinsic biosensors cpT-Sapphire Yellen G
Metabolites

glutamate

FLIP-cpGltI210

FRET-based biosensors Ito T
Metabolites

PtdIns(3,4)P2

Pippi-PI(3,4)P2

FRET-based biosensors MDCK cells Matsuda M
Metabolites

glutamate

FRET glutamine reporter

FRET-based biosensors Ludewig U
Metabolites

PtdIns(3,4)P2

Pippi-PI(3,4)P2

FRET-based biosensors COS-7 cells Matsuda M
Metabolites

1,2-diacylglycerol

Cys1-GFP

Translocation-based biosensors Meyer T
Metabolites

glutamate

FLIP-cpGlnH183

FRET-based biosensors Ito T
Metabolites

PtdIns(3,5)P2

GFP-PHGRP1

Translocation-based biosensors confocal microscopy PC12 cells GFP Cullen PJ
Metabolites

1,2-diacylglycerol

DAGR (FRET)

Translocation-based biosensors Newton AC
Metabolites

histidine

FLIP-HisJ/cpHisJ194

FRET-based biosensors Ito T
Metabolites

PtdIns(3,5)P2

GFP-PHPDK1

Translocation-based biosensors Alessi DR
Metabolites

arabinose

FLIParaF.Ec-250n

FRET-based biosensors spectroscopy Kd-230nM ± 18 nM E. coli BL21-Gold(DE3) E.coli K12 araF eCFP, Venus Frommer WB Available
Metabolites

inositol 1,4,5-trisphosphate

LIBRA

FRET-based biosensors Mikoshiba K
Metabolites

PtdIns(3,5)P2

YFP-PHTAPP1

Translocation-based biosensors Alessi DR
Metabolites

arginine

FRET arginine reporter

FRET-based biosensors confocal flourescence microscopy, spectroscopy pH-sensitive, ornithine Kd-9.2 mM, Arg Kd-3.2 mM E. coli BL21(DE3)Gold, Arabidopsis thalina Protoplasts and roots bacterial glutamine binding protein (QBP) from E. coli with T70A and D157N eCFP, Citrine (shortened) Ludewig U
Metabolites

inositol 1,4,5-trisphosphate

FIRE1-3

FRET-based biosensors Mignery GA
Metabolites

PtdIns(3,5)P2

GFP-Svp1p

Translocation-based biosensors Lemmon MA
Metabolites

amino acids

FLIPs for amino acids

FRET-based biosensors fluorescence microscopy, spectroscopy cross-binding of other aa Yeast cells circularly permutated PBPs eCFP, Venus Ito T
Metabolites

inositol 1,4,5-trisphosphate

IRIS-1

FRET-based biosensors Tojyo Y

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