Filters
Sensor Target Typesort ascending Specificity Sensor Name Sensor Type Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs
Protein Activation and Conformation

Rho family GTPase activation

Raichu-CRIB

FRET-based biosensors Matsuda M
Protein Activation and Conformation

Rho family GTPase activation

Raichu-RhoA

FRET-based biosensors Matsuda M
Protein Activation and Conformation

Rho family GTPase activation

Raichu-RBD

FRET-based biosensors Matsuda M
Protein Activation and Conformation

Rho family GTPase activation

Raichu-RalA

FRET-based biosensors COS-7 cells CFP, YFP Matsuda M
Protein Activation and Conformation

Rho family GTPase activation

RhoA biosensor commentary

FRET-based biosensors Hahn KM, Hahn KM
Protein Activation and Conformation

Wiskott -Aldrich syndrome protein (WASP) conformation

Cdc42-GEF sensors

FRET-based biosensors NIH 3T3 cells eCFP, eYFP Rosen MK
Protein Activation and Conformation

activation of plasma membrane Ca2+ pump

BFP-PMCA-GFP

FRET-based biosensors spectroscopy concentration of BFP-PMCA-GFP-26 nM, PMCA-GFP-30 nM Saccharomyces cerevisiae strain DBY 2062, yeast cells Ca2+-calmodulin binding BFP, GFP Adamo HP
Protein Activation and Conformation

Akt conformation

ReAktion

FRET-based biosensors fluorescence microscopy NIH3T3 fibroblasts human Akt-1/protein kinase B-a eCFP, Citrine Zhang J
Protein Activation and Conformation

Akt conformation

GFP–Akt–YFP

FRET-based biosensors FLIM NIH 3T3 protein kinase B (PKB)/Akt eGFP , eYFP Larijani B
Protein Activation and Conformation

Annexin A4 self-association

CYNEX4

FRET-based biosensors confocal flourescence microscopy, FRAP HeLa cells, N1E-115, MDCK2 neuroblastoma cells Annexin A4 (Ca2+ regulated phospholated) eCFP, eYFP Schultz C
Protein Activation and Conformation

CaMKII conformation

CamuiαamCYCaMIIα

FRET-based biosensors two-photon fluorescence microscopy HeLa cells YFP(Venus) , CFP (S175G) Schultz C, Hayashi Y
Protein Activation and Conformation

ERK conformation

Miu2

FRET-based biosensors HeLa cells, COS-7 cells, 293F CFP, YFP, RFP(Dropna) Matsuda M
Protein Activation and Conformation

Estrogen receptor ligand binding domain conformation

CEY

FRET-based biosensors COS-7 cells, Sf9 cells, Hf7c cells ER-Ligand binding domain seCFP, eYFP Lannigan DA
Protein Activation and Conformation

G protein activation

FP-Gα/FP-Gβ(γ)

FRET-based biosensors HeLa cells, HEK cells, HEK293T, Dictyostelium discoideum cells, FRT cells, haploid yeast cells YFP(Gaq-mYFP) and CFP(mTq), YFP and CFP, eYFP Gadella TW Jr, Lohse MJ, Bünemann M, Gilman AG, Bünemann M, Devreotes P, Scarlata S, Simon MI
Protein Activation and Conformation

Insulin receptor activation

Sinphos

Single FP-based extrinsic biosensors CHO-IR cells, CHO-K1 cells CFP(cpFP), GFP(cpFP) Umezawa Y
Neurotrophic Factor

Neurotrophic factor

BBD-ECat/ECaus (cell-based)

FRET-based biosensors CHO-EGFR cells , MCF-7 cells CFP, YFP Umezawa Y
Metabolites

cAMP

FP-PKARII/FP-PKAcat

FRET-based biosensors eBFP and GFP or CFP and YFP Pozzan T, Pozzan T
Metabolites

Phosphatidylserine

GFP-Lact-C2

Translocation-based biosensors Grinstein S
Metabolites

PtdIns(4,5)P2

PHOSBP

Translocation-based biosensors confocal microscopy COS-7 cells GFP, mRFP Balla T, Munro S
Metabolites

cAMP

CFP–Epac–YFP

FRET-based biosensors fluorescence microscopy highly sensitive cAMP indicator, Kd of 2.3 µM human A431 cells, Rat-1 and NIH3T3 fibroblasts, mouse GE11 epithelial cells, mouse N1E-115 neuroblastoma, human MCF7 breast carcinoma cells cAMP binding CFP, YFP Jalink K
Metabolites

PtdIns(3,4,5)P3

GFP-ARNO

Translocation-based biosensors Czech MP
Metabolites

PtdIns(4,5)P2

GFP-PHFAPP

Translocation-based biosensors confocal microscopy HeLa cells, yeast cells eGFP Lemmon MA
Metabolites

cAMP

ICUEs

FRET-based biosensors eCFP, Citrine Zhang J, Zhang J, Lefkowitz RJ
Metabolites

PtdIns(3,4,5)P3

GFP-PHGRP1

Translocation-based biosensors confocal microscopy PC12 cells GFP Cullen PJ, Czech MP
Metabolites

PtdIns(4,5)P2

CAY

FRET-based biosensors Swiss 3T3 fibroblasts Allen PG

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