Filters
Sensor Target Type Specificity Sensor Namesort descending Sensor Type Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs Addgene Link
Ions

Ca2+

FIP-CBSMTN-XL

FRET-based biosensors Miyawaki A, Persechini A,, Ikura M
Metabolites

inositol 1,4,5-trisphosphate

FIRE1-3

FRET-based biosensors Mignery GA
Redox and Voltage

voltage

Flare

Single FP-based extrinsic biosensors Sakai R, Isacoff EY
Redox and Voltage

voltage

FlaSh

Single FP-based extrinsic biosensors Isacoff EY, Sakai R, Isacoff EY
Redox and Voltage

voltage

FlaSh-CFP/YFP

FRET-based biosensors salamander retinal cones, on-bipolar cells, horizontal cells, wide-field amacrine cells eGFP, GFPuv, and wtGFP, eCFP and eYFP Isacoff EY
Metabolites

cGMP

FlincG

Single FP-based extrinsic biosensors Dostmann WR
Metabolites

glutamate

FLIP-cpGlnH183

FRET-based biosensors Ito T
Metabolites

glutamate

FLIP-cpGltI210

FRET-based biosensors Ito T
Metabolites

histidine

FLIP-HisJ/cpHisJ194

FRET-based biosensors Ito T
Metabolites

leucine

FLIP-LivJ/cpLivJ261

FRET-based biosensors Ito T
Metabolites

arabinose

FLIParaF.Ec-250n

FRET-based biosensors spectroscopy Kd-230nM ± 18 nM E. coli BL21-Gold(DE3) E.coli K12 araF eCFP, Venus Frommer WB Available
Metabolites

glutamate

FLIPE sensors

FRET-based biosensors confocal flourescence microscopy, spectroscopy KD 630 nM Rat hippocampal cells, PC12 cells glutamate/aspartate binding protein ybeJ from Escherichia coli eCFP, Venus Frommer WB, Frommer WB
Metabolites

glucose

FLIPglu sensors

FRET-based biosensors confocal flourescence microscopy, spectroscopy KD 600µM Saccharomyces, arabidopsis, HEK293T, COS-7 cells mature glucose/galactose-binding protein MglB eCFP, eYFP Frommer WB, Frommer WB, Frommer WB
Metabolites

maltose

FLIPmal sensors

FRET-based biosensors confocal flourescence microscopy arabinose FLIP Kd-200 nM,maltose FLIP Kd-40 µM Escherichia coli BL21-Gold(DE3) cells periplasmic binding protein for arabinose or maltose, E. coli high-affinity L-arabinose binding protein AraF eCFP, eYFP (venus) Frommer WB
Ions

PO42-

FLIPPi

FRET-based biosensors confocal flourescence microscopy Kd - 30mM COS-7 cells, CHO cells, E. coli BL21(DE3)Gold cells Synechococcus phosphate-binding protein (PiBP) eCFP, Venus Frommer WB Available
Metabolites

ribose

FLIPrib sensors

FRET-based biosensors Lalonde S
Metabolites

amino acids

FLIPs for amino acids

FRET-based biosensors fluorescence microscopy, spectroscopy cross-binding of other aa Yeast cells circularly permutated PBPs eCFP, Venus Ito T
Metabolites

sucrose

FLIPsuc sensors

FRET-based biosensors Frommer WB
Metabolites

tryptophan

FLIPW sensors

FRET-based biosensors Frommer WB
Metabolites

PtdIns(3,4,5)P3

Fllip

FRET-based biosensors CHO cells Umezawa Y
Protein Activation and Conformation

G protein activation

FP-Gα/FP-Gβ(γ)

FRET-based biosensors HeLa cells, HEK cells, HEK293T, Dictyostelium discoideum cells, FRT cells, haploid yeast cells YFP(Gaq-mYFP) and CFP(mTq), YFP and CFP, eYFP Gadella TW Jr, Lohse MJ, Bünemann M, Gilman AG, Bünemann M, Devreotes P, Scarlata S, Simon MI
Metabolites

PtdIns(4,5)P2:Ins(1,4,5) P3 dynamics

FP-PHPLCδ1(FRET)

Translocation-based biosensors Meyer T
Metabolites

cAMP

FP-PKARII/FP-PKAcat

FRET-based biosensors eBFP and GFP or CFP and YFP Pozzan T, Pozzan T
Metabolites

PtdIns(4,5)P2:Ins(1,4,5) P3 dynamics

FP-Tubby

Translocation-based biosensors Meyer T
Metabolites

arginine

FRET arginine reporter

FRET-based biosensors confocal flourescence microscopy, spectroscopy pH-sensitive, ornithine Kd-9.2 mM, Arg Kd-3.2 mM E. coli BL21(DE3)Gold, Arabidopsis thalina Protoplasts and roots bacterial glutamine binding protein (QBP) from E. coli with T70A and D157N eCFP, Citrine (shortened) Ludewig U

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