Filters
Sensor Target Type Specificity Sensor Namesort descending Sensor Type Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs Sequence
Ions

Ca2+

Case12/16

Single FP-based extrinsic biosensors Tsien RY, Chudakov DM, Campbell RE
Metabolites

PtdIns(3,4)P2

CAY

FRET-based biosensors Swiss 3T3 fibroblasts Allen PG
Metabolites

PtdIns(4,5)P2

CAY

FRET-based biosensors Swiss 3T3 fibroblasts Allen PG
Metabolites

PtdIns(3,4,5)P3

CAY

FRET-based biosensors Swiss 3T3 fibroblasts Allen PG
Ions

Cd2+

Cd-FRET-2

FRET-based biosensors Merkx M
Protein Activation and Conformation

Wiskott -Aldrich syndrome protein (WASP) conformation

Cdc42-GEF sensors

FRET-based biosensors NIH 3T3 cells eCFP, eYFP Rosen MK
Protein Activation and Conformation

Estrogen receptor ligand binding domain conformation

CEY

FRET-based biosensors COS-7 cells, Sf9 cells, Hf7c cells ER-Ligand binding domain seCFP, eYFP Lannigan DA
Ions

pH

CFP-YFP tandem

FRET-based biosensors Schaefer M
Metabolites

cAMP

CFP–Epac–YFP

FRET-based biosensors fluorescence microscopy highly sensitive cAMP indicator, Kd of 2.3 µM human A431 cells, Rat-1 and NIH3T3 fibroblasts, mouse GE11 epithelial cells, mouse N1E-115 neuroblastoma, human MCF7 breast carcinoma cells cAMP binding CFP, YFP Jalink K
Metabolites

cGMP

cGES sensors

FRET-based biosensors confocal flourescence microscopy HEK-293 cells GAF-B domain of PDE2A and GAF-A domain of PDE5A eCFP, eYFP Lohse MJ
Metabolites

cGMP

cGi sensors

FRET-based biosensors spectroscopy, fluorescence microscopy cGMP concentration 1-2 µM HEK293 cells cGMP-binding sites cNMP-BD (cGKI) or GAF (PDE5) eCFP, eYFP Koesling D
Metabolites

cGMP

CGYs

FRET-based biosensors confocal flourescence microscopy CHO-K1 cells, HEK-293 cells PKG-Ia binding eCFP, eYFP Umezawa Y
Metabolites

citrate

CIT sensors

FRET-based biosensors fluorescence microscopy Kd range - 8 µM - 1765 µM Kd: CFP-CitA6-130-Venus - 8 µM COS cells, arabidopsis, yeast, e.coli citrate binding of the Klebsiella pneumoniae histidine sensor kinase CitA venus, CFP Zamboni N
Metabolites

diacylglycerol

CKAR

FRET-based biosensors fluorescence microscopy, spectroscopy Sensitive to calyculcin A and PKC HeLa cells, MDCK cells FHA2 domain of Rad53p mCFP, mYFP Newton AC
Kinase Activity

Protein kinase C

CKAR; KCP-1

FRET-based biosensors Schultz C, Newton AC
Ions

Cl-

Cl-sensor

FRET-based biosensors fluorescence microscopy, spectroscopy pH-specific/sensitive CHO-K1 cells, HEK cells, hippocampal neurons, photoreceptors from rat retina peptide linker mutated eYFP, CFP Bregestovski P Plain text icon test_sequence_ bregestovski.txt
Ions

Cl-

Clomeleon

FRET-based biosensors confocal flourescence microscopy PKD1 (K467A) Kd: .196 µM, WT PKD1 - .012, µM, PKD1 (K467R) - .009 µM E.coli, HEK-293 cells PDK1 PH domain green fluorescent protein (GFP-) tagged versions of wild-type PDK1 van Aalten DM
Metabolites

BAI2 (a furanosyl borate diester)

CLPY

FRET-based biosensors fluorescence microscopy 98 kDa (CLPY), BAI2 Kd 270 nM BL21 (luxS-) cells transformed with pQE30-CLPY or LuxP-mutant constructs LuxP-BAI2 binding CFP, YFP Sayre R
Ions

Zn2+

CLY9-2His

FRET-based biosensors Merkx M
Redox and Voltage

redox (superoxide)

cpYFP

Single FP-based intrinsic biosensors confocal microscopy human osteosarcoma cells cpYFP Cheng H
Kinase Activity

Abl

Crk-based reporter

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific (tyrosine) NIH 3T3, MEF cells complete chicken c-CrkII gene eCFP, eYFP (citrine) Tsien RY
Kinase Activity

Protein kinase A

CRY-AKAR

FRET-based biosensors HeLa cells Cerulean, mVenus, mCherry Zhang J
Redox and Voltage

redox

CY-RL5 and CY-RL7

FRET-based biosensors CFP and eYFP, mCyPet and mYPet Gaskins HR, Gaskins HR
Kinase Activity

CyclinB-Cdk1

CyclinB-Cdk1 sensor

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase Specific HeLa cells, Xenopus, MEF, hTert-RPE1 cells Polo box domain (PBD) of PIk1 CFP(mCerulean) , YFP(YPet) Pines J
Metabolites

cGMP

Cygnets

FRET-based biosensors confocal flourescence microscopy cGMP Kd - 600 nM, cAMP Kd - 300 µM rat aortic smooth muscle cells, Sf9 insect cells, RFL-6 fibroblast cells PKG-Ia binding eCFP, Citrine Dostmann WR

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