Filters
Sensor Target Type Specificity Sensor Name Sensor Type Analysis Technique IV Propertiessort descending Organism Binding Protein Fluorophore PIs Addgene Link
Metabolites

glutamate

FLIP-cpGlnH183

FRET-based biosensors Ito T
Redox and Voltage

voltage

Flare

Single FP-based extrinsic biosensors Sakai R, Isacoff EY
Metabolites

PtdIns(3,5)P2

GFP-PHGRP1

Translocation-based biosensors confocal microscopy PC12 cells GFP Cullen PJ
Metabolites

1,2-diacylglycerol

Cys1-GFP

Translocation-based biosensors Meyer T
Metabolites

BAI2 (a furanosyl borate diester)

CLPY

FRET-based biosensors fluorescence microscopy 98 kDa (CLPY), BAI2 Kd 270 nM BL21 (luxS-) cells transformed with pQE30-CLPY or LuxP-mutant constructs LuxP-BAI2 binding CFP, YFP Sayre R
Metabolites

maltose

FLIPmal sensors

FRET-based biosensors confocal flourescence microscopy arabinose FLIP Kd-200 nM,maltose FLIP Kd-40 µM Escherichia coli BL21-Gold(DE3) cells periplasmic binding protein for arabinose or maltose, E. coli high-affinity L-arabinose binding protein AraF eCFP, eYFP (venus) Frommer WB
Metabolites

ATP

ATeams

FRET-based biosensors spectroscopy, fluorescence microscopy ATeams Kd - 7.4 µM and 1.7 (AT3.10), 14 µM and 2.0 (AT3.10MGK), 1.2 mM and 2.1 (AT1.03YEMK), and 3.3 mM and 2.1 (AT1.03), respectively. HeLa cells, NIH 3T3 ? subunit from Bacillus subtilis FoF1-ATP synthase CFP (mseCFP), YFP (cp173-mVenus) Noji H
Metabolites

BAI2 (a furanosyl borate diester)

LuxP-FRET

FRET-based biosensors spectroscopy, fluorescence microscopy BAI2 Kd 270 nM Escherichia coli BL21 (luxS-), V. harVeyi strains BB120 (wild-type), MM30 (luxS-), MM32 (luxS-, luxN-) LuxP-BAI2 binding CFP, YFP Sayre R
Metabolites

cAMP

(T)Epac(VV)

FRET-based biosensors FLIM, confocal fluorescence microscopy cAMP indicators Hek293 cells, N1E-115, human HeLa cells (ccl-2) cAMP binding mTurquise (mECFPD-Epac(CD, and DEP)-cp173Venus-Venus Jalink K
Metabolites

cAMP

HCN2-camps

FRET-based biosensors confocal flourescence microscopy, FRAP cAMP-sensitive HEK 293a cells, mouse heart single cAMP binding of HCN2 channel- a helices covering cAMP binding site eCFP, eYFP Engelhardt S
Metabolites

cGMP

cGi sensors

FRET-based biosensors spectroscopy, fluorescence microscopy cGMP concentration 1-2 µM HEK293 cells cGMP-binding sites cNMP-BD (cGKI) or GAF (PDE5) eCFP, eYFP Koesling D
Metabolites

cGMP

Cygnets

FRET-based biosensors confocal flourescence microscopy cGMP Kd - 600 nM, cAMP Kd - 300 µM rat aortic smooth muscle cells, Sf9 insect cells, RFL-6 fibroblast cells PKG-Ia binding eCFP, Citrine Dostmann WR
Protein Activation and Conformation

activation of plasma membrane Ca2+ pump

BFP-PMCA-GFP

FRET-based biosensors spectroscopy concentration of BFP-PMCA-GFP-26 nM, PMCA-GFP-30 nM Saccharomyces cerevisiae strain DBY 2062, yeast cells Ca2+-calmodulin binding BFP, GFP Adamo HP
Metabolites

amino acids

FLIPs for amino acids

FRET-based biosensors fluorescence microscopy, spectroscopy cross-binding of other aa Yeast cells circularly permutated PBPs eCFP, Venus Ito T
Ions

Cu+

Ace1-FRET

FRET-based biosensors fluorescence microscopy, spectroscopy Cu+ sensitive Yeast cells, E.coli Copper (I) binding domain CFP, YFP He C
Ions

Cu+

Mac1-FRET

FRET-based biosensors fluorescence microscopy, spectroscopy Cu+ sensitive Yeast cells, E.coli Copper (I) binding domain CFP, YFP He C
Ions

Cu+

Amt1-FRET

FRET-based biosensors fluorescence microscopy, spectroscopy Cu+ sensitive, Kd for Cu+ binding: 2.5x10^-18 M E.coli, yeast cells, CHO-K1 cells Copper binding domain of Amt1 CFP, YFP He C
Metabolites

cAMP

CFP–Epac–YFP

FRET-based biosensors fluorescence microscopy highly sensitive cAMP indicator, Kd of 2.3 µM human A431 cells, Rat-1 and NIH3T3 fibroblasts, mouse GE11 epithelial cells, mouse N1E-115 neuroblastoma, human MCF7 breast carcinoma cells cAMP binding CFP, YFP Jalink K
Kinase Activity

ATM-Protein Kinase

ATOMIC

FRET-based biosensors confocal flourescence microscopy irreversible HeLa cells, NIH 3T3, AT4Bi Rad53 FHA2 (Chk2) and peptide with T68 phosphorylation site CFP, YFP Hunter T
Ions

PO42-

FLIPPi

FRET-based biosensors confocal flourescence microscopy Kd - 30mM COS-7 cells, CHO cells, E. coli BL21(DE3)Gold cells Synechococcus phosphate-binding protein (PiBP) eCFP, Venus Frommer WB Available
Metabolites FRET-Based sensor confocal microscopy Kd 1: 8 uM/ Kd 2: 800 uM HeLa cells, Astrocytes, HEK293 cells, hippocampal neurons LldR mTFP, Venus Felipe Barros Available
Metabolites

glucose

FLIPglu sensors

FRET-based biosensors confocal flourescence microscopy, spectroscopy KD 600µM Saccharomyces, arabidopsis, HEK293T, COS-7 cells mature glucose/galactose-binding protein MglB eCFP, eYFP Frommer WB, Frommer WB, Frommer WB
Metabolites

glutamate

FLIPE sensors

FRET-based biosensors confocal flourescence microscopy, spectroscopy KD 630 nM Rat hippocampal cells, PC12 cells glutamate/aspartate binding protein ybeJ from Escherichia coli eCFP, Venus Frommer WB, Frommer WB
Metabolites

citrate

CIT sensors

FRET-based biosensors fluorescence microscopy Kd range - 8 µM - 1765 µM Kd: CFP-CitA6-130-Venus - 8 µM COS cells, arabidopsis, yeast, e.coli citrate binding of the Klebsiella pneumoniae histidine sensor kinase CitA venus, CFP Zamboni N
Metabolites

arabinose

FLIParaF.Ec-250n

FRET-based biosensors spectroscopy Kd-230nM ± 18 nM E. coli BL21-Gold(DE3) E.coli K12 araF eCFP, Venus Frommer WB Available

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